Sequencing by Hybridization (SBH) is a method for reconstructing a DNA sequence based on its k-mer content. This content, called the spectrum of the sequence, can be obtained from hybridization with a universal DNA chip. The main shortcoming of SBH is that it reliably reconstructs only sequences of length at most square root of the size of the chip. Frieze et al. [9] showed that by using gapped...

Detecting recurrent mutations in the BRCA1 and BRCA2 genes presents a challenge because these mutations are scattered throughout each of the genes. An efficient mutation detection system for these genes should have the properties of a scanning methodology in combination with the fidelity of DNA sequence analysis. We have evaluated an Arrayed Primer EXtension (APEX) methodology applied to the id...

Genetic regulation Central dogma of molecular biology: DNA makes RNA makes protein. Gene regulatory data are primarily from ChIP-chip and ChIP-seq. BioTapestry is an interactive tool for building, visualizing, and simulating genetic regulatory networks. Hamid Bolouri, a Hutch member, is an author. Transcription factors bound to DNA perform transcription regulation Small RNAs: miRNA (micro RNA) ...

Figure 1: The procedures of the proposed method. In the technology of DNA chips and cDNA microarrays, there is a great deal of studies on composing oligo DNA on the DNA chip and the cDNA microarray by the present situation, but there are a few studies on designing oligo DNA sequences, which are fixed on the DNA chip and the cDNA microarray [4]. Probe design is the essential procedure for high-t...

The development of miniaturised devices for genetic diagnosis is of great interest for medicine applications. In this paper, we focus on the design, the experimental technology and characterization of a new silicon micro-bio-chip for rapid testing of DNA material. This chip integrates two classical processes for DNA analysis: polymerase chain reaction – PCR – technique for fragments amplificati...

This organ-on-a-chip device of the outer blood retinal barrier will allow future studies complex disease mechanisms and treatments visual disorders using clinically relevant endpoints in vitro.

Electrical discharge machining process is one of the most Applicable methods in Non-traditional machining for Machining chip in Conduct electricity Piece that reaching to the Pieces that have good quality and high rate of machining chip is very important. Due to the rapid and widespread use of alloy DIN1.2080 in different industry such as Molding, lathe tools, reamer, broaching, cutting guillot...

High-occupancy target (HOT) regions are the segments of the genome with unusually high number of transcription factor binding sites. These regions are observed in multiple species and thought to have biological importance due to high transcription factor occupancy. Furthermore, they coincide with house-keeping gene promoters and the associated genes are stably expressed across multiple cell typ...

We describe a high-throughput method, named ChIP-SNP, for the identification of allele-specific protein-DNA interactions throughout the human genome. ChIP-SNP combines chromatin immunoprecipitation (ChIP) with whole-genome single nucleotide polymorphism (SNP) genotyping microarray analysis. We demonstrated that it can be used to accurately identify allele-specific binding of RNA polymerase II (...

Interactions between DNA and proteins located in the cell nucleus play an important role in controlling physiological processes by specifying, augmenting and regulating context-specific transcription events. Chromatin immunoprecipitation (ChIP) is a widely used methodology to study DNA-protein interactions and has been successfully used in various cell types for over three decades. More recentl...