This paper reports experiments on the stereospecificity observed in the monophenolase and diphenolase activities of mushroom tyrosinase. Several enantiomorphs of monophenols and o-diphenols were assayed: L-tyrosine, D,L-tyrosine, D-tyrosine; L-alpha-methyltyrosine, D,L-alpha-methyltyrosine; L-dopa, D,L-dopa, D-dopa; L-alpha-methyldopa, D,L-alpha-methyldopa; L-isoprenaline, D,L-isoprenaline and ...

Enzyme kinetics are usually described by the Michaelis-Menten equation, where the time-dependent decrease of substrate (-dS/dt) is a hyperbolic function of maximal velocity (Vmax), Michaelis constant (Km), and amount of substrate (S). Because the Michaelis-Menten function in its most general meaning requires an assumption of steady-state, it is less curvilinear than true enzyme kinetics. A satu...

The ability of structural analogues of ascorbate to serve as substitutes for this reducing agent in the prolyl 4-hydroxylase reaction was studied. In experiments using the purified enzyme, variations of the compounds' side chain were compatible with co-substrate activity. The presence of very large hydrophobic substituents or a positively charged group caused an increase in the observed Km valu...

Management of asymptomatic patients with severe aortic stenosis (AS) is subject to debate. A watchful waiting strategy is adopted for the vast majority of asymptomatic patients because the surgical risk exceeds the spontaneous risk. However, a subset of patients with asymptomatic severe AS might benefit from early surgery. Only 1 study reported that asymptomatic patients with very severe AS (VS...

Ureases are amidohydrolases that catalyze the hydrolysis of urea to ammonia and carbamate. In addition enzymatic function, ureases have fungitoxic insecticidal which independent their catalytic activity. Soy (Glycine max) has two main urease isoforms: ubiquitous embryo-specific, latter is present in beans. view potential applications ureases, this work aimed extract, purify, characterize struct...

Endothelial nitric oxide synthase (NOS) protein and mRNA have been identified and calcium-dependent NOS activity has been measured in human placentae during normal pregnancy. Recently, mRNA and protein for the inducible isoform of NOS have been detected in placentae of women with gestational diabetes. The aim of this study was to determine whether calcium-independent (ciNOS) and/or total (tNOS)...

A rise in [K+]o, by depolarizing the resting membrane potential and partially inactivating the inward Na+ current (INa), is believed to play a critical role in slowing conduction during myocardial ischemia. In multicellular ventricular preparations, elevation of [K+]o has been suggested to decrease Vmax to a greater extent than expected from membrane depolarization alone. The mechanism of this ...

We calculate the distribution of the distance headways (i.e., the instantaneous gap between successive vehicles) as well as the distribution of instantaneous distance between successive jams in the Nagel-Schreckenberg (NS) model of vehicular traffic. When the maximum allowed speed, Vmax, of the vehicles is larger than unity, over an intermediate range of densities of vehicles, our Monte Carlo (...

The development of the Na/H antiporter was studied in renal brush border membrane vesicles (BBMV) from fetal and adult rabbits using isotopic and fluorescent techniques. The kinetics of the antiporter studied by 22Na+ uptake revealed that the Vmax was only 25% of that in the adult; however, the Km's for Na+ were not significantly different. These data were confirmed by a fluorescent assay using...