Wheat α-amylase, a multi-domain protein with immense industrial applications, belongs to α+β class of proteins with native molecular mass of 32 kDa. In the present study, the pathways leading to denaturation and the relevant unfolded states of this multi-domain, robust enzyme from wheat were discerned under the influence of temperature, pH and chemical denaturants. The structural and functional...

Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments. Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ra...

background: enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-d-galactoside galactohydrolase, ec 3.2.1.23), trivial called lactase. orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. metho...

understanding of physiological responses of salt tolerance might be lead to identify main salt tolerance mechanisms and essential approaches for improving grain yield stability, and also to select salt tolerant wheat cultivars. thus, the effects of salinity were investigated on grain yield and some of the characteristics related to salt tolerance. six wheat cultivars (roshan, kavir, karchia, ga...

BACKGROUND Improving the hydrolytic performance of xylanolytic enzymes on arabinoxylan is of importance in the ethanol fermentation industry. Supplementation of debranching (arabinofuranosidase) and depolymerizing (xylanase) enzymes is a way to address the problem. In the present study, we identified a bifunctional α-l-arabinofuranosidase/endo-xylanase (Ac-Abf51A) of glycoside hydrolase family ...

this study investigated the influence of inoculation with an arbuscular mycorrhizal fungus (amf), glomus mosseae (nicol & gerd.) on growth and antioxidant enzyme activities (sod, cat, gupx) in shoots and roots of wheat (triticum aestivum l.) affected by three different levels of salt stress. the experiment was arranged as a factorial in randomized complete block design (rcbd) with three replicat...

  Objective(s): Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed.   Materials and Methods: Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much ...

This study was conducted to investigate the effects of thyme essential oil and enzyme supplementation on rations with two different energy (wheat or corn) protein resources (soy rapeseed meals) gut morphology digestibility broiler chickens. Three hundred eighty-four male Ross-308 chickens were used a 23 factorial arrangement treatments according completely randomized design. The morphological e...

Cereal Chem. 69(5):542-546 The changes in rheological properties of a wheat flour dough due to the wheat flour dough were clearly influenced both by the type of the addition of enzymes were measured. The dough, made of flour of a-amylase and by mixing protease with the a-amylase. The presence of standard baking quality, was characterized by oscillatory measurements a-amylases caused a decrease ...