For many years, two-dimensional gel electrophoresis has been the method of choice for the investigation of complex mixtures of proteins. Although there are a number of emerging technologies that can be applied to proteomics, none can yet yield routinely the breadth of information available from two-dimensional gels. To be able to obtain instant information regarding molecular mass and pI, as we...

Two-dimensional gel electrophoresis (2D-GE) employs isoelectric focusing in the first dimension and a separation of the proteins according to their molecular weights in the second dimension. The gels are then commonly stained using silver stain to visualize the protein pattern. This is a tedious and time-consuming procedure. Here, we demonstrate a combination of two newly developed methods – OF...

The mobilities of oligomers of phage lambda DNA and of yeast chromosomes in agarose gels during field inversion gel electrophoresis (FIGE) were measured at different pulse times and electric fields. Also the ratios between forward and backward pulse times and/or field gradients were varied. The problem of 'band inversion' during FIGE, leading to an ambiguity in the mobility of large DNA fragmen...

Dose-related effects of methapyrilene (MP) on protein modification and expression were examined using two-dimensional gel electrophoresis (2-D PAGE) coupled with computer analysis. Methapyrilene was administered ad libitum at doses of 0, 62.5, 125, 250 and 1000 p.p.m. to male F-344 rats for 12 weeks beginning at 8 weeks of age. Following treatment, livers were removed and frozen for 2-D PAGE an...

Multiwavelength detection of laser induced fluorescence for dideoxynucleotide DNA sequencing with four different fluorophores and separation by capillary gel electrophoresis is described. A cryogenically cooled, low readout noise, 2-dimensional charge-coupled device is used as a detector for the on-line, on-column recording of emission spectra. The detection system has no moving parts and provi...

Fibrinogen synthesis was investigated in guinea pig megakaryocytes. Purified megakaryocytes were incubated with 35S-methionine in methionine-free incubation medium for 18 hours. Newly synthesized fibrinogen in megakaryocyte lysates enriched with purified carrier guinea pig fibrinogen was immunoprecipitated with a specific anti-guinea pig fibrinogen antiserum produced in rabbits. Proteins in the...