نتایج جستجو برای: 2 region as well as ttg 49 microsatellite primer
تعداد نتایج: 7513607 فیلتر نتایج به سال:
A database of 30,137 EST sequences from Mycosphaerella graminicola, the septoria tritici blotch fungus of wheat, was scanned with a custom software pipeline for di- and trinucleotide units repeated tandemly six or more times. The bioinformatics analysis identified 109 putative SSR loci, and for 99 of them, flanking primers were developed successfully and tested for amplification and polymorphis...
Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Here we describe microsatellite discovery on a Pacific Biosciences single molecul...
Primer approximation multiplex PCR (PAMP) is a new experimental protocol for efficiently assaying structural variation in genomes. PAMP is particularly suited to cancer genomes where the precise breakpoints of alterations such as deletions or translocations vary between patients. The design of PCR primer sets for PAMP is challenging because a large number of primer pairs are required to detect ...
Sorghum is the fifth degree of importance in the production of cereal after wheat, rice, maize and barley. Genetic diversity estimation of plant material is primary step for identification, protection and conservation of germplasm and breeding programs designing. In order to study of genetic diversity among 10 genotypes of grain sorghum, 10 microsatellite primers were used according to previous...
this study attempts to investigate the effect of peers’ revision in comparison to that of the teacher, and whether peers’ comments and teachers’ comments facilitate students’ revision? if yes, which one is more effective? also attempts have been made to see which aspects of language are more highlighted by peers versus teachers when commenting. besides, it is investigating the student’s attitud...
it is not possible to understand developments of a country’s culture and civilization without knowing his believes and myths. whether it is north or south, east or west, planes or mountains, sahara or deserts, here in iran every corner includes valuable and precious treasures which are known all over the world, and cultural – historical objects which are found here adorn all well known museums...
SRC kinases and PKR are intracellular protein kinases, which play key roles in intracellular viral replication. In this research, the effect of SRC kinase inhibition and PKR activation and inhibition on replication of coxsakievirus (CVB3), an entrovirus of the family picornaviridae – causative agents of fatal myocarditis, was studied. Vero and Hela cells were cultured and infected with CVB3 in ...
Ig gene rearrangement by Ig complementarity determinig region 3 (CDR3) spectratyping . Total RNA was extracted using RNeasy Minikit (Quiagen) or Nucleo Spin-RNA/protein (Macherey-Nagel) columns and was retrotranscribed with Advantage RT for PCR kit (Clontech). cDNA was amplified with the following fast performance liquid chromatography (FPLC) purified primers (Invitrogen): mu1 forward (FW), CAG...
dendrimer synthesis strategies now provide virtual control of macromolecular nanostructures as a function of size and surface/interior functionality. these strategies involve the covalent assembly of hierarchical components reactive monomers , branch cells or dendron’s around atomic or molecular cores according to divergent/convergent dendrite branching principles.combinon of two or more dendri...
هدف تحقیق حاضر بررسی تاثیر نشانه های فرا گفتمان متنی بر فهم متون انگلیسی به وسیله ی زبان اموزان فارسی زبان است.این تحقیق علاوه بر این کوشیده است تا میزان اگاهی این زبان اموزان و نحوه ی تعامل انان را با متون خوانده شده در زبان انگلیسی به وسیله ی پرسش نامه ی تهیه شده بررسی کند.بر اساس محتوای یک متن انگلیسی یازده سوال درست /غلط طرح گردید و یک مرتبه با ان متن و یک مرتبه با نسخه ای که نشانه ها...
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