Histone chaperones are a group of proteins that aid in the dynamic chromatin organization during different cellular processes. Here, we report that the human histone chaperone nucleophosmin interacts with the core histones H3, H2B, and H4 but that this histone interaction is not sufficient to confer the chaperone activity. Significantly, nucleophosmin enhances the acetylation-dependent chromati...

Chemically induced histone-DNA crosslinking in nuclei is used to monitor structural changes in chromosomal domains containing hyperacetylated histones. Core particles harbouring the crosslinks are immunofractionated with antibodies specific for acetylated histones. Crosslinking is revealed by gel separation of tryptic peptides from core histones that carry 32P-labelled residual nucleotide. The ...

The effects of konjac glucomannan (KGM) on thermal behavior of wheat starch have been studied in the presence of low concentrations of Na2CO3 (0.1-0.2 wt% of starch). Confocal laser scanning microscopy (CLSM) allows the visualization of the starch gelatinization process and granule remnants in starch pastes. Heating the starch dispersion in KGM-Na2CO3 solution significantly delays granule swell...

Cationic waxy corn starch was prepared from waxy corn starch with 2,3epoxypropyl trimethyl ammonium chloride (ETMAC) as a cationic etherifying reagent. Its structure was identified by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray powder diffraction (XRD). The results showed that quaternary ammonium groups were introduced successfully into the waxy...

PURPOSE The RAS association domain family protein 1 (RASSF1) has been implicated in a tumor-suppressive function through the induction of acetylated α-tubulin and modulation of cell migration. However, the mechanisms of how RASSF1A is associated with acetylation of α-tubulin for controlling cell migration have not yet been elucidated. In this study, we found that RASSF1A regulated cell migratio...

Sago starch is a locally grown and produced starch resource in Malaysia. In this study, corn starch to sago starch ratios (CS:SS) of 0:20, 5:15, 10:10, 15:5 and 20:0 were included in feed mixture to contain 20% starch and produce five isonitrogenous and isocaloric (30% crude protein and 16.7 kJ/g, respectively) tilapia Oreochromis sp. diets. Diets were preconditioned to contain 40% moisture and...

this article reports a study of the thermo-oxidation and biodegradation in the soil of low-density polyethylene/starch films (ldpe/starch) by infrared spectroscopy: films of ldpe/starch blends of different composition (starch content range from 0 to 15% w/w),were prepared and exposed to thermo-oxidation at different temperatures at 80, 90 and 100°c,and buried in the soil of public dump during 3...

Sago starch is a locally grown and produced starch resource in Malaysia. In this study, corn starch to sago starch ratios (CS:SS) of 0:20, 5:15, 10:10, 15:5 and 20:0 were included in feed mixture to contain 20% starch and produce five isonitrogenous and isocaloric (30% crude protein and 16.7 kJ/g, respectively) tilapia Oreochromis sp < em>. diets. Diets were preconditioned to contain 40% moistu...

An important issue for chromatin remodeling complexes is how their bromodomains recognize particular acetylated lysine residues in histones. The Rsc4 subunit of the yeast remodeler RSC contains an essential tandem bromodomain (TBD) that binds acetylated K14 of histone H3 (H3K14ac). We report a series of crystal structures that reveal a compact TBD that binds H3K14ac in the second bromodomain an...