A fibrinogen fragment obtained by limited-plasmin proteolysis has been isolated and purified to apparent homogeneity by gel filtrations. This fragment, denoted as 24-Kd fragment, has an apparent M, -24,000 and contains an N-terminal sequence of met-glu-leu-glu-arg-pro-gly-glyasn-glu-ile. The fragment contains a large number of acidic amino acid residues, and its amino acid composition is simila...

A novel Ca2+ binding protein, named caligulin, was extracted from the heat-treated 100 000 X g supernatant of bovine brain and purified to electrophoretic homogeneity. The apparent Mr of caligulin determined on sodium dodecyl sulfate polyacrylamide gels was 24000. Analysis by gel filtration chromatography indicated an apparent Mr of 33000, suggesting a monomeric protein. Amino acid composition ...

This article reports an experimental and theoretical investigation of mercury dissolution from dental amalgams immersed in neutral (noncorrosive) and acidic (corrosive) flows. Atomic absorption spectrophotometric measurements of Hg loss indicate that in neutral flow, surface oxide films formed in air prior to immersion persist and effectively suppress significant mercury release. In acidic (pH ...

Human milk oligosaccharides (HMO) are considered to influence the composition of the gut microflora in breastfed infants. We investigated direct effects of milk HMO fractions or individual oligosaccharides on proliferation, differentiation and apoptosis in transformed human intestinal cells (HT-29 and Caco-2) and non-transformed small intestinal epithelial crypt cells of fetal origin (human int...

The catalytic esterification of n-propyl propionate using propionic acid and n-propyl alcohol as reactants via a conventional pilot plant reactive distillation column was carried-out. The strongly acidic ion-exchange resin Amberlyst46TM was used as the solid acid catalyst. The influence of the reflux ratio (from 2.0 to 2.5), the molar feed ratio (from 2.0 to 2.5), and total feed (from 3.0 to 4....

A fibrinogen fragment obtained by limited-plasmin proteolysis has been isolated and purified to apparent homogeneity by gel filtrations. This fragment, denoted as 24-Kd fragment, has an apparent M, -24,000 and contains an N-terminal sequence of met-glu-leu-glu-arg-pro-gly-glyasn-glu-ile. The fragment contains a large number of acidic amino acid residues, and its amino acid composition is simila...

Two glycoproteins were purified and biochemically characterized from the lichen X. parietina. Both behaved as enzymes with arginase activity and haemaglutinins. Secreted arginase (SA) contained galactose and glucose in the saccharide moiety and an isoelectric point of 4.54. The algal binding-protein (ABP) had N-acetyl-glucosamine and glucose as glycosidic residues and an isoelectric point of 3....

A trypsin inhibitor was isolated from beans of Phaseolus vulgaris, cultivar. Kintoki, and the specific activity increased 200 times as high as that of the crude extract. It was homogeneous on several electrophoreses and the molecular weight was about 13,000. The amino acid composition was characterized by high ratios of cystine, aspartic acid, and serine. It inhibited trypsin in a molar ratio o...