The molecular weight (MW) of a protein can be predicted based on its amino acids (AA) composition. However, in many cases a non-chemically modified protein shows an SDS PAGE-displayed MW larger than its predicted size. Some reports linked this fact to high content of acidic AA in the protein. However, the exact relationship between the acidic AA composition and the SDS PAGE-displayed MW is not ...

BACKGROUND The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and...

The composition of the major acidic extracellular polysaccharide (EPS) of 25 strains of Rhizobium japonicum was determined. Eight strains synthesized an acidic EPS containing rhamnose and 4-O- methylglucuronic acid and were closely related according to DNA homology. These same strains also expressed high levels of ex planta nitrogenase activity. Sixteen strains produced an acidic EPS containing...

The evidence for a regulatory role of acidic isoferritins on hemopoiesis is not entirely consistent with our knowledge of ferritin biochemistry, and no clear picture of this phenomeonon has emerged. In the present study, we have been unable to confirm a consistent effect of purified heart (acidic), spleen (basic), or serum (glycosylated) isoferritins on CFU-GM colony formation in vitro. Inhibit...

Acidic phospholipid binding plays an important role in determining the tissue distribution of basic drugs. This article describes the use of surface plasmon resonance to measure binding affinity (KD) of three basic drugs to phosphatidylserine, a major tissue acidic phospholipid. The data are incorporated into mechanistic tissue composition equations to allow prediction of the steady-state volum...

in this study, the oil yield, fatty acid (fa) composition and the physicochemical and quality characteristics of salvia macrosiphon crude seed oil were determined. the main fatty acids ranked in the following order of abundance: α- linolenic acid> linoleic acid> oleic acid. the n3 /n6 fa ratio and polyunsaturated fa/ saturated fa ratio were 1.6 and 7.23, respectively. the fa composition reveale...