1. The lipoproteins of the liver cell sap can be resolved by paper electrophoresis into five components. Almost two-thirds of the total lipid, cholesterol and phospholipid are present in the slowest- and the fastest-moving components. 2. There is a two- to three-fold increase in the lipid content of the liver cell sap after the administration of carbon tetrachloride. 3. The cholesterol and phos...

sites on the polysaccharide using simple sugars as prospective inhibitors. Although much discussion is given to this topic, a really critical evaluation of the method as it is applied would have been welcome. As if overcome by long hours of work in purifying and assaying, the members of the conference several times lapse into rather unrewarding philosophical speculation on the question of wheth...

Although analysis strategies exist for probing a diverse array of molecular properties, most of these approaches are not amenable to the study of reaction intermediates and other transient species. Separations in particular can provide detailed information on attributes not readily measured by spectroscopy but typically are performed over time scales much longer than the life span of highly uns...

with 5% acetic acid and, after 10 min, the gel was finally washed in distilled water and air-dried onto the glass support. Air-drying allows the long and thin gel to be easily submitted to photographic and/or digital recording using a sequencing gel-sized scanner, such as the HP ScanJet 4C/T (Hewlett-Packard, Palo Alto, CA, USA). This avoids wethandling or the need of an apparatus for gel-dryin...

Hemoglobin (Hb) Porto Alegre is a beta globin chain mutant [beta 9 (A6) Ser>Cys] that was initially described in a Caucasian Brazilian family in 1963(1). It was subsequently identified in other families in Brazil and in other places such as Cuba and the Canary Islands. The origin of the mutation was reported as Portuguese by the co-inheritance of an intragenic polymorphism characteristic of the...

Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating saccharides. Oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16 h under the given experimental conditions. Saccharide-ANTS adducts were then separated...

Tears and skin detachments were observed in a newborn calf after umbilical revision. The presumptive diagnosis of Dermatosparaxis was based on the anamnesis typical clinical signs disease, such as high fragility total detachment fragments. Three staining techniques (HE, PAS Masson’s trichrome) performed samples from affected animal, using same region normal animal with age crossbreed, ranch use...

bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. the aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...