Intrauterine devices block luteolysis in cyclic mares, but the underlying mechanism is unknown. To clarify mechanisms, protein profile of endometrial secretome was analyzed using two-dimensional difference gel electrophoresis (2D-DIGE). Twenty-seven mares were classified according to whether they inseminated (AI) or had an intrauterine device (IUD), a water-filled plastic sphere, inserted into ...

The goal of this work was the research chondroitin sulfate prophylactic action on glutathione system state in rat blood serum during acute joint inflammation. performed white non-linear pubertal males weighting 160-240 grams compliance to all general ethical principles animal research. All animals were divided four experimental groups. first group – control: injected with 0,1 ml 0,9 % NaCl solu...

There are various undesirable products generated from endogenous aerobic metabolism such as reactive oxygen species (ROS). Physiological and biochemical lesions caused by ROS which give rise to oxidative damages towards DNA, proteins lipids ultimately lead cell death. This study was aimed examine the effect of oral administration food colorants (tartrazine curcumin) on oxidants antioxidants lev...

Abstract We developed a computational pipeline to study immunometabolism for drug discovery in immune mediated diseases. Our integrates RNA-seq and proteomics data build genome scale metabolic models integrate these with external databases patient perform flux analyses identifying targets repurposing. applied our explore the metabolism of B cells context rheumatoid arthritis (RA) systemic lupus...

Genetically engineered enzymes of modified structure are proved very effective biocatalysts for many biosyntheses of industrial interest, which tend more and more to replace some of the classical fine chemical synthesis processes. Engineered enzymatic reactions, displaying a high selectivity and specificity, are sustainable bioengineering routes to obtain a wide range of biochemical products in...

Determination of the N-terminal sequences of two EPTC (S-ethyl dipropylcarbamothioate)-induced proteins from thiocarbamate-degrading Rhodococcus sp. strain NI86/21 resolved by two-dimensional electrophoresis enabled the localization of the respective structural genes on two distinct DNA fragments. One of these strongly induced proteins is a NAD(+)-dependent dehydrogenase active on aliphatic ald...

PURPOSE We studied whether the accumulation of advanced lipoxidation end-products (ALEs) in the diabetic retina is linked to the impairment of lipid aldehyde detoxification mechanisms. METHODS Retinas were collected from nondiabetic and diabetic rats and processed for conventional and quantitative RT-PCR (qRT-PCR), Western blotting, immunohistochemistry, and aldehyde dehydrogenase (ALDH) acti...