RWRWRW-NH2 (MP196) is an amphipathic hexapeptide that targets the bacterial cytoplasmic membrane and inhibits cellular respiration and cell wall synthesis. In previous studies it showed promising activity against Gram-positive bacteria and no significant cytotoxicity or hemolysis. MP196 is therefore used as lead structure for developing more potent antibiotic derivatives. Here we present a more...

In this study, we determined the maximum tolerated plasma concentration of suramin (within the predetermined study target range) when combined with doxorubicin in the treatment of androgen-independent prostate cancer. Twenty-four patients received suramin dosages based on proportional adjustment of the steady-state plasma suramin concentration to achieve the targeted plasma concentrations of 50...

To establish a method for determining ursolic acid content in Patrinia scabiosifolia Fisch. ex Trevir.; and to investigate the inhibitory effect of Patrinia scabiosifolia Fisch. ex Trevir. extract on in vitro cultured human cervical carcinoma Hela cells. HPLC with Agilent XBP-C18 column (4.6 mm × 250 mm, 5 μm) was used. Mobile phase was acetonitrile-methanol-0.5% ammonium acetate solution (68:1...

Methods: Fifteen extended-spectrum b-lactamaseor carbapenemase-producing E. coli were studied. The in vitro pharmacodynamic model was inoculated with 1×10 cfu/mL and ertapenem was dosed once daily at 0 and 24 h to simulate free ( f ) Cmax and t12 obtained after either 1 g or 2 g intravenous once-daily doses in healthy volunteers (1 g: f Cmax 15 mg/L, t12 4 h). Sampling was performed over 48 h t...

Aims To study loop diuretic response and effect of omission in ambulatory heart failure (HF) patients on chronic low-dose diuretics. Methods results Urine collections were performed two consecutive days 40 HF with 40–80 mg furosemide (day 1 diuretic; day 2 without diuretic). Three phases collected each day: (i) first 6 h; (ii) rest the day; (iii) night. On intake, total natriuresis was 125.9 (8...

Abstract Background Management of patients treated with Ticagrelor is challenging, as stopping prior to coronary bypass graft surgery (CABG) may increase the risk acute stent thrombosis. The aim study was compare bleeding complications in ticagrelor combined acetylsalicylic acid (ASA) versus ASA alone until 1 day before surgery. Methods Bleeding complications, defined composite red blood cell t...

Background: Methotrexate (MTX) is one of the frequently used chemotherapeutic agents, especially in hematological malignancies and solid tumors. Objectives: MTX hepatotoxicity symptoms range from elevations in serum aminotransferases to hepatocyte necrosis and fibrosis. The time of medication administration significantly impacts treatment outcomes. Hence this study evaluated the protective eff...

OBJECTIVE To study whether quantitative analysis of M. tuberculosis mRNA could be used to assess bacterial viability. METHODS The levels of M. tuberculosis mRNA were compared 24, 48, and 72 hours after M. tuberculosis H(37)R(V) was treated with no drug, 1.0 micro g/ml and 10.0 micro g/ml rifampicin, 0.2 micro g/ml and 1.0 micro g/ml isoniazid, 2 micro g/ml and 4 micro g/ml ethambutol, 2 micro...

Introduction: The Serum/Glucose deprivation -induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. Nigella sativa L. has been known as a source of antioxidants. To elucidate the neuroprotective actions of N. sativa extract in vitro, we studied the effect of N. sativa extract on cultured PC12 cells under s...

Fumonisins represent a family of toxic, structurally related metabolites produced by fungi that are found in corn worldwide. We investigated the effects of the mycotoxin, fumonisin B(1), on rat splenic macrophage and lymphocyte functions. Pretreatment (24 h) of resident macrophages with fumonisin B(1) (1, 10, and 100 microg/ml) significantly (p<0.01) stimulated nitric oxide production (0.48, 2....