نتایج جستجو برای: bacmid shuttle vector

تعداد نتایج: 203458  

F. Moradian, G. Rahimi H. Oghbatalab H. Rahimian

Rumen bacterial strains can potentially be manipulated to perform functions different from wild type species. The most numerous species of bacteria in the rumen and gut are species of the familyBacteroidetes, whichcan have the potential for genetic modification for enzyme production. One of the genetic manipulation of rumen bacteria can perform for production of starch digestive enzyme for the ...

Ali Akbar Shaebani, Delavar Shahbazzadeh, Fereidoun Mahboudi, Mohsen Karimi, Robert W. McMaster,

Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...

2009
Jianshuang Wang Yinsheng Wang

Humans are exposed to N-nitroso compounds from both endogenous and exogenous sources. Many N-nitroso compounds can be metabolically activated to give diazoacetate, which can result in the carboxymethylation of DNA. The remarkable similarity in p53 mutations found in human gastrointestinal tumors and in shuttle vector studies, where the human p53 gene-containing vector was treated with diazoacet...

Journal: :Nucleic acids research 1995
M S Bradshaw J A Bollekens F H Ruddle

The functional analysis of genes frequently requires manipulation of large genomic regions embedded in yeast artificial chromosomes (YACs). We have designed a yeast-bacteria shuttle vector, pClasper, that can be used to clone specific regions of interest from YACs by homologous recombination. The important feature of pClasper is the presence of the mini-F factor replicon. This leads to a signif...

Journal: :jundishapur journal of microbiology 0
manoochehr makvandi infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran ali teimoori infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; research center for infectious diseases of digestive system; imam khomeini hospital, ahvaz jundishapur university of medical sciences, ahvaz, ir iran niloofar neisi infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9166136984, fax: +98-6133332036; +98-6133362411 alireza samarbafzadeh infectious and tropical disease research center, health research institute, department of virology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions the results of the present study showed that the baculovirus expression system (sf9 cells) was able to express truncated orf2 and truncated orf2-nsp4 proteins as a potential candidate vaccine. background the hepatitis e virus (hev) accounts for hepatitis e infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. the baculovirus expression...

2014
Sachin Telang Poonam Patel Vishwas Sarangdhar Sheela Donde

With the aim of engineering a strain of bacteria that could be used for bioremediation of cellulosic waste in radioactive environments, the gene for the secreted endoglucanase enzyme of Bacillus pumilis was decided to be cloned into the radiotolerant bacterium, Deinococcus radiodurans. The endoglucanase gene from B. pumilus was PCR amplified and cloned into Escherichiacoli DH5α using a pDrive v...

Journal: :BioTechniques 2006
Qi Ma Li Zhou Lixin Ma Keke Huo

The baculovirus expression vector system (BEVS) has become one of the most widely used systems for routine protein expression. We have developed an improved strategy to clone foreign genes directionally and directly into the baculovirus genome vector via a one-step procedure to generate recombinant viruses in a week. In this work, we constructed a host strain Escherichia coli DH10BacHB1.1, whic...

Journal: :Journal of virology 2014
Ming Yang Shuo Wang Xiu-Li Yue Lu-Lin Li

UNLABELLED Autographa californica multiple nucleopolyhedrovirus orf132 (named ac132) has homologs in all genome-sequenced group I nucleopolyhedroviruses. Its role in the viral replication cycle is unknown. In this study, ac132 was shown to express a protein of around 28 kDa, which was determined to be associated with the nucleocapsids of both occlusion-derived virus and budded virus. Confocal m...

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