conclusions in general, it seems that three-month exercise training was effective in reducing cardiac mitochondrial pro-apoptotic protein. however, considering the results of the bcl2 gene expression, more researches are needed to identify effects of exercise trainings on indices of myocardial apoptosis. objectives the aim of this study was to assess the effect of exercise training on bcl-2 and...

Under certain conditions, T4 gene 32 protein is known to increase the efficiency of different enzymes, such as Taq DNA polymerase, reverse transcriptase, and telomerase. In this study, we compared the efficiency of the SMART PCR cDNA synthesis kit with and without the T4 gene 32 protein. The use of this cDNA synthesis procedure, in combination with T4 gene 32 protein, increases the yield of RT-...

abstract during the winter and spring of 2007 and 2008, 450 samples (leaves of carnation plants) showing symptoms of yellowing, mottling, leaf malformation, and stunting were collected from greenhouses in mashhad and chenaran regions. the samples were tested by das-elisa for the presence of carmv. total rna was extracted by rnxtm (-plus) solution from positive infected samples confirmed in das-...

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyz...

Here, we describe a suppression PCR-based method for directional cloning of randomly primed cDNAs from small quantities of tissue. Synthesis of the first cDNA strand is conducted on oligonucleotide-coated magnetic beads. Synthesis of the second strand is accomplished using nonspecifically primed suppression PCR. This method is used to synthesize a cDNA library from zebrafish embryos at 6-9 h af...

We review different methodologies to estimate the expression levels of microRNAs (miRNAs) using real-time quantitative PCR (qPCR). As miRNA analysis is a fast changing research field, we have introduced novel technological approaches and compared them to existing qPCR profiling methodologies. qPCR also remains the method of choice for validating results obtained from whole-genome screening (e.g...

background: polycystic ovary syndrome (pcos) is related to obesity and to major metabolic alterations including both insulin resistance and beta-cell dysfunction. ghrelin was identified as the endogenous ligand for the growth hormone secretagogue (ghs) receptor. the actions of ghrelin are carried out through interaction with specific receptor, named ghs-r. objective: in a case-control study, we...

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...