In recent years, combination therapy has emerged as the cornerstone of clinical practice in treating glioblastoma multiforme. However, their ability to trigger and leverage body's adaptive immunity rarely been studied. Tumour heterogeneity, presence blood-brain barrier, an immunosuppressive tumor microenvironment play a crucial role 90% local recurrence post-treatment. Herein, we report improve...

Follicular dendritic cells (FDCs) are stromal cells residing in primary follicles and in germinal centers of secondary and tertiary lymphoid organs (SLOs and TLOs). There, they play a crucial role in B-cell activation and affinity maturation of antibodies. FDCs have the unique capacity to bind and retain native antigen in B-cell follicles for long periods of time. Therefore, FDCs shape the B-ce...

Autologous T cells were genetically modified to express a chimeric antigen receptor (CAR) directed toward carboxy-anhydrase-IX (CAIX) and used to treat patients with CAIX-positive metastatic renal cell carcinoma. In this study, we questioned whether the T cell maturation stage in the pre-infusion product affected CAIX CAR expression and function in vitro as well as in vivo CAR T cell numbers an...

When T cells are primed by dendritic cells (DCs) to initiate antigen-specific immune responses screening for matching antigen receptor-MHC/peptide pairs takes place in DC-T-cell conjugates. For an immune response DC-T-cell conjugates formed during priming events need to dissolve. Although detailed knowledge on molecules involved in the conjugate formation is available, dissolving of them has no...

The signals controlling the checkpoints of dendritic cells (DC) maturation and the correlation between phenotypical and functional maturational stages were investigated in a defined model system of growth factor-dependent immature mouse DC. Three sequential stages of DC maturation (immature, mature, and apoptotic) were defined and characterized. Immature DC (stage 1) had low expression of costi...

Abstract Regulatory T cells (Tregs) are crucial in inducing and maintaining tolerance, already have shown clinical potential. In this study, we describe an efficient non-viral engineering method by TCR-encoding mRNA electroporation, while also exploring the potential of further promoting on-target effects disrupting endogenous TCR using a fully RNA-based CRISPR/Cas9 technology. Endogenous disru...

Abstract Immunity to infections is crucial healthy life. However, recent Covid-19 pandemic has shown that comorbidity such as kidney disease and associated uremia impairs immunity against infections. Uremic patients show high susceptible also exhibit poor antibody responses vaccine. The mechanisms by which negatively impacts response unknown. Using multiple mouse models of uremia, we assessed B...

Background: Cryopreservation of ovaries is an important technique in assisted reproduction technology. Physical forces like hydrostatic pressure have a pivotal role in reproduction systems. Due to changes in intrafollicular pressure during ovulatory process, this study designed to examine the effects of hydrostatic pressure on oocyte maturation and cell death in cumulus cells from cryopreserved...