background: sellick's maneuver (cricoid pressure) may make laryngoscopic view and tracheal intubation more difficult. this study compared the different techniques of cricoid pressure (cp) regarding to the laryngoscopic view and time to intubation. methods: in142 anesthetized patients, 4 types of cp was randomly applied; a) one-handed cp using thumb, index and middle fingers, b) one-handed cp us...

Monoclonal antibody CKM-G01 inhibited greater than 99% of the activity of porcine and human creatine kinase(CK)-MM isoenzyme purified from muscle. However, it inhibited only 54% of CK-MM in human serum. Chromatofocusing of serum CK-MM showed that CKM-G01 inhibited 100% of MM3 but not isoform MM1. CKM-G01 inhibited CK-MM2 by 57%. CKM-G01 specifically inhibited only the original CK-M subunit and ...

Background Cyclophosphamide (CP) is a chemotherapeuticdrug which causes deleterious effects on tissue of testicle and increases free radicals in the body. The aim of this study is to investigate protective effects of ethyl pyruvate (EP) on improvement of testicle inCP treated animals. MaterialsAndMethods In this study, 15 male mice(6-8 weeks) were divided into 3 groups. Control group receivedno...

A new commercial kit (Impres-MB; International Immunoassay Labs.) recently was introduced for measuring the MB isoenzyme of creatine kinase (CK-MB) based on the use of monoclonal antibodies. After antibodies to CK-MM isoenzyme are added to precipitate the CK-MM, antibodies to CK-M monomer are added to precipitate the M-subunit isoenzymes of CK. Subtracting the enzymatic activity of the second s...

Merkel cell carcinomas (MCCs) are uncommon, highly malignant skin tumors that develop in sun-exposed areas of the skin. Most of the MCCs are CK 20-positive and CK 7-negative such as our case. About 80% of Merkel cell carcinoma is associated with Merkel cell polyomavirus.

Fusion of splenocytes from A/J mice immunized by creatine kinase (EC 2.7.3.2)-MB isoenzyme (CK-MB) with SP2/0-Ag14 myeloma cell line generated hybridomas producing monoclonal antibodies specific to CK-MB. One of these monoclonal antibodies ("Conan-MB") was used to develop a direct assay for CK-MB activity. In the assay, serum is incubated for 30 min at room temperature with "Conan-MB" immobiliz...

The effect of i.v. nitroglycerin administration on indexes of infarct size was examined in 31 patients with acute myocardial infarction. Serial creatine kinase (CK) and CK-MB isoenzyme determinations were used to calculate infarct size. Twenty-nine patients served as controls. Two subgroups of the study group were formed to evaluate differences between early and late intervention. In the first ...

BACKGROUND Creatine kinase (CK) exists as three cytosolic isoenzymes, CK-MM, CK-MB, and CK-BB, and one mitochondrial isoenzyme. Animal and human observations suggest that the CK-MB content of myocardium is dynamic and may increase in response to ischemia, but the response of the myocardial CK system to chronic coronary artery occlusion is not well-defined. METHODS AND RESULTS We measured seri...

cisplatin (cp) as an important anti-tumor drug causes nephrotoxicity mainly by oxidative stress and renin-angiotensin system (ras). since flavonoids have high antioxidant activity and probable role in the inhibition of ras, this study was designed to investigate the protective effect of hydroalcoholic extract and flavonoid fraction of morus alba leaves on cisplatin-induced nephrotoxicity in rat...