ABSTRACT: The present study evaluated the cryoprotectant efficacy of dimethylacetamide (DMA) and ethylene glycol in a one-step protocol to freeze boar sperm. sperm-rich portion ejaculates from two boars were collected once week, for 10 weeks. After collection, diluted (1:1; v/v) cooling extender. determining their spermatozoa concentration, pooled with same number each stabilized at 20°C 120 mi...