نتایج جستجو برای: cryoprotectants

تعداد نتایج: 630  

2012
E. Isachenko P. Mallmann G. Rahimi J. Risopatròn M. Schulz V. Isachenko

The method is cheap and quick. Due to lowering the temperature of solution's glass transition the permeable cryoprotectants prevents the actual freezing of solution and allows to maintain its some flexibility in a glassy phase. Vitrification without permeable cryoprotectants allows to avoid the cryoprotectants toxicity and osmotic stress; the damage of plasmatic and mitochondrial membrane durin...

2015
Joana E. Matos Carla C. Marques Teresa F. Moura Maria C. Baptista Antonio E. M. Horta Graça Soveral Rosa M. L. N. Pereira

BACKGROUND In cryopreservation, oocytes are subjected to extreme hyperosmotic conditions, inducing large volume changes that, along with an abrupt temperature drop, interfere with their developmental competence. Our objectives in this work were to find conditions enabling an increase in oocyte cryosurvival and subsequent development. METHODS Abattoir-derived bovine oocytes were cultured witho...

2016
Fabio Mosca Manuela Madeddu Ahmad Abdel Sayed Luisa Zaniboni Nicolaia Iaffaldano Silvia Cerolini

This data article contains supporting information regarding the research article entitled "Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm"(Mosca et. al., 2016) [1]. The combined effect of the permeant cryoprotectants agent dimethylacetamide and the non-permeant cryoprotectants agent trehalose on the quality of frozen-thawed chicken sem...

2017
Keisuke Edashige

The permeability of the plasma membrane to water and cryoprotectants is one of the important factors for determining the suitable condition for the vitrification of mammalian oocytes and embryos. Water and cryoprotectants move slowly through oocytes and early embryos, principally by simple diffusion, in the mouse, bovine, pig, and human. In contrast, water, glycerol, and ethylene glycerol move ...

Journal: :Crystals 2022

The three-dimensional structure of protein is determined by analyzing diffraction data collected using X-ray beams. However, beam can damage crystals during collection, lowering the quality crystal data. A way to prevent such treating with cryoprotectants. cryoprotectant stabilizes and prevents Many kinds cryoprotectants are commercially available, various treatment methods have also been repor...

2012
Felipe de Lara Janz Adriana de Aguiar Debes Rita de Cássia Cavaglieri Sérgio Aloísio Duarte Carolina Martinez Romão Antonio Fernandes Morón Marcelo Zugaib Sérgio Paulo Bydlowski

Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expres...

Journal: :Animal reproduction science 2009
George Shigueki Yasui Lenin Arias-Rodriguez Takafumi Fujimoto Katsutoshi Arai

The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees C for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (DMSO)...

Journal: :Cryobiology 2013
Vanesa Robles Felipe Martínez-Pastor Giuliano Petroni Marta F Riesco Anna Bozzano Roger Villanueva

Cephalopod culture is expected to increase in the near future and sperm cryopreservation would be a valuable tool to guarantee sperm availability throughout the year and to improve artificial insemination programs. We have studied the tolerance of spermatophores from the oceanic squid Illex coindetii to several cryoprotectants, in two toxicity experiments and a cryopreservation test. Five perme...

2010
M. H Mapeka K. C. Lehloenya T. L. Nedambale B. Sutherland

________________________________________________________________________________ Abstract The study evaluated the effect of different cryoprotectants on post-thaw survival and motility of Kolbroek sperm. Semen from Kolbroek boars was collected with the gloved hand technique. Ejaculates were diluted with Beltsville thawing solution (BTS) at a ratio of 1 : 1 prior to freezing. Semen was diluted w...

2012
Pedro Fonte Sandra Soares Ana Costa José Carlos Andrade Vítor Seabra Salette Reis Bruno Sarmento

PLGA nanoparticles are useful to protect and deliver proteins in a localized or targeted manner, with a long-term systemic delivery pattern intended to last for a period of time, depending on polymer bioerosion and biodegradability. However, the principal concern regarding these carriers is the hydrolytic instability of polymer in aqueous suspension. Freeze-drying is a commonly used method to s...

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