نتایج جستجو برای: direct sample pcr
تعداد نتایج: 971582 فیلتر نتایج به سال:
Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.
The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted of a PCR performed directly on cells after a proteinase K pretreatment (direct PCR), a PCR after purification of nucleic acids (DNA-PCR), and a PCR with rRNA sequences as the target after reverse transcription. The direct P...
BACKGROUND Human pathogens that can cause infertility may also affect sperm count and quality. Viral infections can be considered as direct and/or indirect cause of male factor infertility. OBJECTIVE Our goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men attending the Avicenna Infertility Clinic, and to compare it with the herpes virus serology result...
Standard RANSAC does not perform very well for contaminated sets, when there is a majority of outliers. We present a method that overcomes this problem by transforming the problem into a 2D position vector space, where an ordinary cluster algorithm can be used to find a set of putative inliers. This set can then easily be handled by a modified version of RANSAC that draws samples from this set ...
objective: erythrasma is a chronic superficial infection of the intertriginous areas. most laboratories use methylene blue stain and 10% koh smear to identify corynebacterium minutissimum (c. minutissimum) by direct observation of filamentous bacilli. occasionally atypical forms can be seen that create problems in diagnosis. this study aims to use the polymerase chain reaction (pcr) method in o...
A new PCR kit (AMPLICOR CT/NG; Roche Diagnostic Systems, Inc., Branchburg, N.J.) was used as a screening tool for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine (FVU) specimens from 3,340 asymptomatic women attending European health care units for contraceptive advice or pregnancy termination. All samples were kept frozen (220°C) prior to testing. Chlamydia...
for the first time nakayama introduced qf-ring. in 1967 carl. faith and elbert a. walker showed that r is qf-ring if and only if each injective right r-module is projective if and only if each injective left r-modules is projective. in 1987 s.k.jain and s.r.lopez-permouth proved that every ring homomorphic images of r has the property that each cyclic s-module is essentialy embeddable in dire...
Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing, which is time consuming and labor intensive. In this study, five species-specific (Nm-sodC and ...
background and objectives: the aim of this study was to compare direct microscopic examination with culture and pcr for the diagnosis of mucorales infection in blood and tissue specimens. material and methods: blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. direct smear of tissue samples ...
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