In the current study, hypothesis of effects luteinizing hormone (LH) and follicular fluid (FF) derived from follicles varying size on in vitro embryo production Shami goat breed were tested. The caprine (cFF) was obtained healthy female’s ovaries by aspiration method classified into two main classes (follicles with a diameter ≤ 2mm ≥3mm). resulting cFF added to culture medium TCM-199 through si...

Abstract Study question To evaluate secretion of b-hCG hormone by human post-implantation embryos cultured in-vitro up to day 10 according their chromosomal status. Summary answer Secreted levels differs among with different load, being severely diminished in monosomic when compared either euploid or trisomic embryos. What is known already Exploring early embryo development subject growing inte...

Human sperm cryopreservation for assisted reproduction is compromised by ROS-induced sperm cryodamage. Our previous model study in which mouse sperm were treated with H₂O₂ to simulate sperm DNA-damage caused by cryopreservation-induced ROS have discovered that mouse embryos fertilized with treated sperm showed a delay in cleavage that might be associated with cell cycle arrest. The DNA-damage c...

The p20K gene is induced in conditions of reversible growth arrest in chicken embryo fibroblasts (CEF). This expression is dependent on transcriptional activation and on a region of the promoter designated the quiescence-responsive unit (QRU). In this report, we describe the regulatory elements of the QRU responsible for activation in resting cells and characterize the trans-acting proteins int...

A comprehensive study is presented of a series of 124 infertile men undergoing testicular sperm retrieval for intracytoplasmic sperm injection (ICSI). In this study we correlated the histological changes observed in the testicular tissue with the results of the wet preparation and the outcome after ICSI using testicular spermatozoa. In all patients with normal spermatogenesis and hypospermatoge...

Previously, we reported that breast cancer cells with retinoblastoma (pRb) pathway-defective checkpoints can be specifically targeted with chemotherapeutic agents, following staurosporine-mediated reversible growth inhibition in normal cells. Here we set out to determine if the kinetics of staurosporine-mediated growth inhibition is specifically targeted to the G(1) phase of cells, and if such ...

Once every menstrual cycle, eggs are ovulated into the oviduct where they await fertilization. The ovulated eggs are arrested in metaphase of the second meiotic division, and only complete meiosis upon fertilization. It is crucial that the maintenance of metaphase arrest is tightly controlled, because the spontaneous activation of the egg would preclude the development of a viable embryo (Zhang...