Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...

to evaluate the role of wild promoter of l-phenylalanine dehydrogenase (phedh) gene, referred to as pdh, from bacillus sphaericus in expression, cloning of pdh gene in bacillus subtilis was performed. the whole pdh gene was cloned in phy300plk shuttle vector and amplified, construct (phydh) then transformed in b. subtilis isw1214 and e. coli jm109. the pdh endogenous promoter presented no effec...

By screening 74 chordate genomes for endogenous lentiviruses using Pol sequences of exogenous lentiviruses as a reference, we identified a novel endogenous lentivirus in the genome of the ferret (Mustela putorius furo). Phylogenetic analysis suggested that the ferret endogenous lentivirus, denoted ELVmpf, diverged early in the evolution of the mammalian lentiviruses, although with a lack of res...

The human endogenous retrovirus K (HERV-K) family of endogenous retroviruses consists of approximately 50 proviral copies per haploid human genome. Herein, the HERV-Ks are shown to encode a sequence-specific nuclear RNA export factor, termed K-Rev, that is functionally analogous to the HIV-1 Rev protein. Like HIV-1 Rev, K-Rev binds to both the Crm1 nuclear export factor and to a cis-acting vira...

We have developed RNA expression microarrays (REMs), in which each spot on a glass support is composed of a population of cDNAs synthesized from a cell or tissue sample. We used simultaneous hybridization with test and reference (housekeeping) genes to calculate an expression ratio based on normalization with the endogenous reference gene. A test REM containing artificial mixtures of liver cDNA...

The identification of meat adulteration is a hotspot for food research worldwide. In this paper, a smart and sealed biosensor that combines loop-mediated isothermal amplification (LAMP) with a lateral flow device (LFD) was developed, resulting in the universal mammalian assessment on site. First, the highly specific chromosomal Glucagon gene (Gcg) was chosen as the endogenous reference gene, an...

The aim of this work was to evaluate the relative gene expression levels cytokines IL- 1B, IL-8, IL-12, IFN-γ, IL-4, IL-10 and TGF-β in somatic milk cells French Alpine breed, anestrous goats that were experimentally infected left mammary gland with Staphylococcus chromogenes during lactation peak. Milk samples obtained from both glands for 21 consecutive days post infection. Total RNA extracte...

Characteristic aroma is a well-appreciated feature contributing to tea quality. Although extensive studies have been made investigate biosynthesis and gene expressions during making processes, it remains unclear whether the endogenous volatile postharvest processing contributes quality of tea. To critically evaluate this question, we used same batch fresh leaves produced three different types w...