Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (...

For many languages there are no large, general-language corpora available. Until the web, all but the richest institutions could do little but shake their heads in dismay as corpus-building was long, slow and expensive. But with the advent of the Web it can be highly automated and thereby fast and inexpensive. We have developed a `corpus factory' where we build large corpora. In this paper we d...

Architecting and designing high volume Web sites has changed immensely over the last six years. These changes include the availability of inexpensive Pentium based servers, Linux, Java applications, commodity switches, connection management and caching engines, bandwidth price reductions, content distribution services, and many others. This paper describes the evolution of the best practices wi...

In this work, a simple and economical procedure for the synthesis of spiro-4H-pyranderivatives has been found through the three-component, one-pot condensation of isatin/acenaphthequinone, malononitrile and different reagents including 1, 3-dicrbonyl compounds, naphthol and 4-hydroxycumarin under thermal and solvent-free conditions in the presence ofFe2O<su...

An enzyme-free and label-free fluorescence turn on biosensor for amplified copper(II) ion (Cu(2+)) detection has been constructed based on self-assembled DNA concatamers and Sybr Green I. This assay is simple, inexpensive and sensitive, enabling quantitative detection of as low as 12.8 pM Cu(2+).

A positive-tone 2D direct-write technique that can achieve sub-wavelength patterning by non-linear overlap effects in a conventional polymer system is described. The technique involves relatively inexpensive free-space optics, skips the usual development step, and promises the possibility of a lithographic method that is solvent-free.

The World Wide Web coupled with user friendly Web browsers now provide access to multimedia Web pages in universally accepted formats that can be accessed world wide easily via inexpensive desktop computers. Everyone appears to agree that this technology will revolutionize how students, faculty, researchers, and the public access and use information. Consequently university educators are now en...

Searching and reading the Web is one of the principal methods used to seek out information to resolve problems about technology in general and digital devices in particular. This paper addresses the problem of text mining in the digital devices domain. In particular, we address the task of detecting semantic relations between digital devices in the text of Web pages. We use a Naı̈ve Bayes model ...