نتایج جستجو برای: gradient pcr
تعداد نتایج: 299570 فیلتر نتایج به سال:
A new learning strategy is proposed for training of radial basis functions (RBF) network. We apply two different local optimization methods to update the output weights in training process, the gradient method and a combination of the gradient and Newton methods. Numerical results obtained in solving nonlinear integral equations show the excellent performance of the combined gradient method in ...
Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in the genesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing and HPV vaccination have been aggressively promoted recently, it is crucial to investigate the HPV distribution in Malaysia in order to maximize their cost-effectiveness. This study was therefor...
background: giardia lamblia is one of the most prevalent intestinal flagellate protozoa that infects a wide range of vertebrate hosts causing severe intestinal disorder in children.this study was performed to determine subspecies of g.lamblia by the pcrrflp method, targeting the glutamate dehydrogenase (gdh) locus, in hospitalized children at urmia mutahhari hospital, west azerbaijan province,i...
Laser forming is a thermal forming process which uses laser beam irradiation to produce the desired final forms. In this article, the effect of temperature gradient across Al 6061-T6 aluminum sheets on bending angle is studied. Input parameters including laser power, scan velocity, beam diameter, and sheet thickness are the effective process parameters which influence the temperature gradient....
results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...
introduction: the amount of expression of bax and bcl-2 genes in infertile men’s sperm as well as its association with sperm parameters and dna fragmentation index is an issue which has not been studied yet. in this research, it is assumed that up-regulation of bax and downregulation of bcl-2 are directly associated with sperm dna fragmentation. methods: after obtaining semen samples from the p...
In gradient scaffolds, changes in porosity, pore size or chemical composition occur gradually. Recently, different methods have been applied to create gradient in the scaffolds, but they have some disadvantages such as high cost and control. The main purpose of this research was to fabricate porous gradient scaffolds by a novel, functional, simple, and low-cost method. Two homogenous scaffolds...
Helicobacter species are fastidious bacterial pathogens that are difficult to culture by standard methods. A PCR-denaturing gradient gel electrophoresis (PCR-DGGE) technique for detection and identification of different Helicobacter species was developed and evaluated. The method involves PCR detection of Helicobacter DNA by genus-specific primers that target 16S rDNA and subsequent differentia...
Many molecular biology assays depend in some way on the polymerase chain reaction (PCR) to amplify an initially dilute target DNA sample to a detectable concentration level. But the design of conventional PCR thermocycling hardware, predominantly based on massive metal heating blocks whose temperature is regulated by thermoelectric heaters, severely limits the achievable reaction speed(1). Cons...
Background & Aims : Giardia lamblia is one of the most prevalent intestinal flagellate protozoan that infects a wide range of vertebrate hosts . Giardia is one of the most common intestinal pathogens in children causing sever intestinal disorder and growth retardation. This study was performed to determine subspecies of Giardia lamblia by the PCR-RFLP method, targeting the glutamate dehydro...
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