نتایج جستجو برای: gradient pcr

تعداد نتایج: 299570  

A. Golbabai, M. Mammadov , S. Seifollahi ,

A new learning strategy is proposed for training of radial basis functions (RBF) network. We apply two different local optimization methods to update the output weights in training process, the gradient method and a combination of the gradient and Newton methods. Numerical results obtained in solving nonlinear integral equations show the excellent performance of the combined gradient method in ...

Journal: :Asian Pacific journal of cancer prevention : APJCP 2010
P P Chong N Asyikin M Rusinahayati S Halimatun R Rozita C K Ng W H Wan Hamilton B C Tan N Noraihan A Rohani H Faezah L Latiffah A Maha A R Sabariah

Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in the genesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing and HPV vaccination have been aggressively promoted recently, it is crucial to investigate the HPV distribution in Malaysia in order to maximize their cost-effectiveness. This study was therefor...

Journal: :iranian journal of parasitology 0
khosro hazrati tappeh center for research of cellular and molecular of urmia, urmia university of medical sciences, urmia, iran. gholamreza manafi dept. of medical parasitology, school of medicine, urmia university of medical sciences, urmia, iran. mohammad asgharzadeh dept. of paramedical faculty, school of medicine, tabriz university of medical sciences, tabriz, iran. farideh manafi dept. of medical biochemistry, school of medicine, ardabil university of medical sciences, ardabil, iran.

background: giardia lamblia is one of the most prevalent intestinal flagellate protozoa that infects a wide range of vertebrate hosts causing severe intestinal disorder in children.this study was performed to determine subspecies of g.lamblia by the pcrrflp method, targeting the glutamate dehydrogenase (gdh) locus, in hospitalized children at urmia mutahhari hospital, west azerbaijan province,i...

Amir H. Roohi, H. Moslemi Naeini J. Shahbazi Karami M. Hoseinpour Gollo Sh. Imani Shahabad

Laser forming is a thermal forming process which uses laser beam irradiation to produce the desired final forms. In this article, the effect of temperature gradient across  Al 6061-T6 aluminum sheets on bending angle is studied. Input parameters including laser power, scan velocity, beam diameter, and sheet thickness are the effective process parameters which influence the temperature gradient....

Journal: :jundishapur journal of microbiology 0
parisa mohammadi department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran; department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran. tel: +98-2185692714, fax: +98-2188058912 aida hamidkhani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran ezat asgarani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Journal: :anatomical sciences journal 0
hossein taheri organization mohammad salehi cellular and molecular biology research center, shahid beheshti university of medical sciences and health services, tehran, iran. tarlan eslami arshaghi organizationسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences) majid mosahebi organization

introduction: the amount of expression of bax and bcl-2 genes in infertile men’s sperm as well as its association with sperm parameters and dna fragmentation index is an issue which has not been studied yet. in this research, it is assumed that up-regulation of bax and downregulation of bcl-2 are directly associated with sperm dna fragmentation. methods: after obtaining semen samples from the p...

In gradient scaffolds, changes in porosity, pore size or chemical composition occur gradually. Recently, different  methods have been applied to create gradient in the scaffolds, but they have some disadvantages such as high cost and control. The main purpose of this research was to fabricate porous gradient scaffolds by a novel, functional, simple, and low-cost method. Two homogenous scaffolds...

Journal: :Journal of medical microbiology 2003
Waleed Abu Al-Soud Mads Bennedsen Stephen L W On Ibn-Sina Ouis Peter Vandamme Hans-Olof Nilsson Asa Ljungh Torkel Wadström

Helicobacter species are fastidious bacterial pathogens that are difficult to culture by standard methods. A PCR-denaturing gradient gel electrophoresis (PCR-DGGE) technique for detection and identification of different Helicobacter species was developed and evaluated. The method involves PCR detection of Helicobacter DNA by genus-specific primers that target 16S rDNA and subsequent differentia...

2011
Radha Muddu Yassin A. Hassan Victor M. Ugaz

Many molecular biology assays depend in some way on the polymerase chain reaction (PCR) to amplify an initially dilute target DNA sample to a detectable concentration level. But the design of conventional PCR thermocycling hardware, predominantly based on massive metal heating blocks whose temperature is regulated by thermoelectric heaters, severely limits the achievable reaction speed(1). Cons...

اصغرزاده , محمد, حضرتی تپه, خسرو , دیبا , کامبیز , غیبی, شاه صنم , محمدزاده, حبیب, منافی , غلامرضا ,

    Background & Aims : Giardia lamblia is one of the most prevalent intestinal flagellate protozoan that infects a wide range of vertebrate hosts . Giardia is one of the most common intestinal pathogens in children causing sever intestinal disorder and growth retardation. This study was performed to determine subspecies of Giardia lamblia by the PCR-RFLP method, targeting the glutamate dehydro...

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