The homophilic potential emerges as an important biological principle to boost the potency of immunoglobulins. Since homophilic antibodies in human and mouse sera exist prior environmental exposure, they are part of the natural antibody repertoire. Nevertheless, hemophilic properties are also identified in induced antibody repertoire. The use of homophilicity of antibodies in the adaptive immun...

We used immunoelectron microscopic localization techniques to investigate whether platelets stimulated by ADP or ristocetin in the plasma milieu bind von Willebrand factor (vWF) to their surfaces. We found by both peroxidase- and ferritin-based methods that unstimulated platelets lack vWF on their surfaces, whereas platelets that are stimulated with ADP or ristocetin have vWF associated with th...

People with hemophilia were formerly at very high risk of infection with hepatitis C virus (HCV). Approximately 20% of HCVinfected patients spontaneously clear the virus. To identify correlates of spontaneous clearance of HCV, we studied a cohort of HCV-infected hemophilic subjects without human immunodeficiency virus infection who had never been treated with interferon. Plasma HCV RNA was pers...

We have used immunoblotting of purified factor VIII (FVIII) to determine whether or not changes in FVIII chain specificity occur during the course of an inhibitor. Serial plasma samples from 15 inhibitor patients (13 hemophilic and two spontaneous) were analyzed. Nine of the 15 antibodies, all with epitopes on the 44-kilodalton (Kd) thrombin fragment of the 92-Kd FVIII heavy chain and/or the 72...

To gain greater insight into the nature of the bleeding tendency in hemophilia, we compared the spatial dynamics of clotting in platelet-free plasma from healthy donors and from patients with severe hemophilia A or B (factor VIII:C or IX:C<1%). Clotting was initiated via the intrinsic or extrinsic pathway in a thin layer of nonstirred plasma by bringing it in contact with the glass or fibroblas...

The objectives of this article were to study the reported prevalence of haemophilia B (HB) on a country-by-country basis and to analyse whether the prevalence of HB varied by national economy. The prevalence of HB is the proportion of diagnosed, reported cases of HB in a population at a specific point of time. We collected data on the HB prevalence for 105 countries from the World Federation of...

The purified factor VIII-related protein we acrylamide-gel electrophoresis, and imhave previously characterized from normal munoprecipitation assays. Likewise, the cryoprecipitate possesses both procoaguprocoogulant and vWf activities are delant activity and vWf activity. We have ficient. As vWf activity is one of the majes attempted to isolate and characterize this biologic functions of either...

1. 19 strains of non-hemolytic hemophilic bacilli were studied. 17 required the addition of V and X growth accessory factors to their media, 2 required the addition of only V. 2. Of 15 strains of hemolytic hemophilic bacilli examined, 10 were found to require the addition of only V to their media, 3 the addition of V and X in the form of yeast extract and hematin, and 2 the addition of accessor...