Background: IgA serum autoantibodies against tissue transglutaminase (tTG) have an established diagnostic value in coeliac disease, and high efficacy tests are widely available for their detection. However, serological evaluation of IgA deficient subjects is still difficult. Aims: To evaluate the diagnostic potential of IgG class anti-tTG autoantibodies measured quantitatively using an enzyme l...

Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is a potentially fatal autoimmune syndrome in which IgG antibodies target the NR1 subunit of NMDA resulting profound dysregulation and neurotransmission.

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

Background and Aims: Hepatitis E virus is a public health concern and about a third of people in the world live in endemic areas. Although HEV is believed to be transmitted by fecal-oral routes between humans in low sanitary conditions, but evidence of transmission have been reported in hemodialysis (HD) patients. Materials and Methods: Aim of present study was to determine the prevalence of a...

Abstract The SARS-CoV-2 pandemic has accelerated vaccine development and testing, an important step in its eradication. Health workers were included among the first subjects to be vaccinated, therefore it was necessary check their immunological status after second dose of vaccine. Serum samples belonging 24 health undergoing vaccination for (Pfizer-BioNTech) analysed: 2 them also possible obtai...

BACKGROUND IgA serum autoantibodies against tissue transglutaminase (tTG) have an established diagnostic value in coeliac disease, and high efficacy tests are widely available for their detection. However, serological evaluation of IgA deficient subjects is still difficult. AIMS To evaluate the diagnostic potential of IgG class anti-tTG autoantibodies measured quantitatively using an enzyme l...

OBJECTIVES Leprosy household contacts represent a group at high risk of developing the disease. The aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters. METHODS Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in lepr...

BACKGROUND Helicobacter pylori (H. pylori), is a common infection in pregnant women accompanied by variations in the levels of the IgM, IgA and IgG antibody isotypes. The variations of anti-H. pylori antibodies during and after pregnancy, and the extent of protection they provide to the mother and the fetus are not completely understood. OBJECTIVES To investigate the changes of the anti-H. py...

Intact human erythrocytes were cross-linked with glutaraldehyde (GA) or dimethyladipimidate (DMA) and tested for their ability to bind [125I]-IgG anti-Rh0(D) and to undergo antibody-mediated hemagglutination. There was no decrease in antibody binding after treatment with GA concentrations up to 1.25% and DMA concentrations up to 1%. Red cells treated with these concentrations of GA and DMA did ...

Background and Aims: Non-Alcoholic Fatty Liver Disease (NAFLD) represents an increasing cause of liver disease worldwide. Cardiovascular (CVD) is the leading morbidity mortality in these patients. Although NAFLD pathophysiology not fully understood alterations fat metabolism seem to play a role. Autoantibodies against apolipoprotein A-1 (anti-apoA-1 IgG) are novel cardiovascular risk factor whi...