improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. the aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different precompacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. the in vitr...

background: ovarian tissue transplantation is emerging technologies for fertility preservation. in addition, in vitro maturation (ivm) of oocytes retrieved from ovarian tissues may overcome the fertility defects in certain cases. objective: the aim was to evaluate the best site for ovarian tissue transplantation in mice. also, feasibility of ivm of oocytes retrieved from auto grafted ovarian ti...

a wide range of biological activities of garlic in vitro and in vivo have been verified including its antioxidant, antitumor and anti-inflammatory effects. indoleamine 2,3-dioxygenase (ido) is an enzyme widely distributed in mammals and is inducible preferentially by ifn-γ. ido degrades the essential amino acid tryptophan to form n-formyl kynurenine. in the present in vitro study, the modulator...

moringaceae, a monogeneric family in capparales (glucosinolate-containing species), includes 14 species. one of them is moringa peregrina (forssk.) fiori., a small tree, which grows in south east of iran. volatile constituents of seed coat and stem of m. peregrina were determined by gc and gc/ms. moreover, extracts of seed and different cultured cells were analyzed by tlc and gc. three volatile...

stem cells are self-renewing cells that can be differentiated into other cell types. conventional in vitro models for studying stem cells differentiation are usually preformed in two-dimensional (2d) cultures. the design of three-dimensional (3d) in vitro models which ideally are supposed to mimic the in vivo stem cells microenvironment is potentially useful for inducing stem cell derived tissu...

The effects of glia conditioned media on the survival and differentiation of embryonic neuron cultures of the Central Nervous System is described. We established glial cultures of peritumoral areas and the culture medium was changed serially and collected as glia conditioned medium (GCM). Neuron enriched cultures plated on poly-L-lysine coated Petri dishes and after 4 days in vitro, the neurona...

Background: The treatment of choice for hydatidosis as an important zoonotic disease is surgery. Different agents are injected into the cyst to prevent secondary hydatidosis. To avoid the side effects of such protoscolicidal agents, considering the high protoscolicidal effects of the garlic extract, we conducted the present study on protoscolices in limited applicable times and compared the ext...