Este trabalho aborda a caracterização epistemológica inerente à estrutura proposicional definida, problematizada e justificada por Kant como “juízo sintético priori”. Inicialmente, o problema é especificado, partir da argumentação de na primeira edição Crítica razão pura, assim no interlúdio dos Prolegômenos e, fim, sua versão definitiva textos Progressos metafísica segunda pura. Depois, são ap...

In the framework of his transcendental philosophy Kant strictly separates morality from aesthetics. The pleasure in the good and the pleasure in the beautiful are two different kinds of pleasure (zwei Arten des Wohlgefallens). As a consequence, a moral act as such cannot be beautiful. Only in a second step does Kant indicate possible connections between morality and aesthetics in his comments o...

Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded β-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting and stabilizing the DNA. In eukaryotes the ssDNA-binding protein (SSB) is known as replication protein A (RPA) and consists of three distinct subuni...

A gene encoding an endopeptidase from Streptococcus parasanguis FW213 has been cloned and shown to have high sequence homology to genes encoding mammalian metalloendopeptidases. The gene, designated S. parasanguis pepO, was cloned into the pET28a expression vector, resulting in a fusion of vector sequences encoding a hexahistidine tag at the carboxyl terminus. The recombinant PepO (rPepO) was e...

We previously showed that a fungal protein, p15, induces neurite outgrowth and differentiation of rat pheochromocytoma PC12 cells. We report here the identification and characterization of a protein similar to p15, found in Streptomyces coelicolor A3(2). This hypothetical protein, tentatively named Scp15, has significant similarity with p15, including conserved positions of four cysteine residu...

Fluorescent indicators that allow real-time imaging of Zn(II) in living cells are invaluable tools for understanding Zn(II) homeostasis. Genetically encoded sensors based on fluorescence resonance energy transfer between fluorescent protein domains have important advantages over synthetic probes. We discovered that hexahistidine tags have a strong tendency to dimerize upon binding of Zn(II) in ...

Working within frameworks drawn from the writings of Immanuel Kant, Alfred Schutz, and Kenneth Burke, this article examines the role that diagnostic categories play in autobiographical accounts of illness, with a special focus on chronic disease. Four lay diagnostic categories, each with different connections to formal medical diagnostic categories, serve as typifications to make sense of the w...

The case of Kant's Critique of Judgment offers a powerful example of the radical disruption of the metaphysical text, enacted by Bataille's major сoncepts. The analysis of the metaphor of economy in Kant, Bataille and Derrida suggests the crucial importance of Bataille's general economy—as the economy of loss—for deconstructing the Kantian conception of genius and the whole scheme of taste—as a...