نتایج جستجو برای: mercaptoethanol tests samples with anti

تعداد نتایج: 9483164  

Journal: :Applied and environmental microbiology 1984
J L Corbin

The analytical potential of the reaction of ammonia with o-phthalaldehyde mercaptoethanol reagent at pH 7 (an atypical fluorescence) has already been demonstrated. This, coupled with additional findings reported here, has led to an ammonia determination well suited to nitrogenase studies. As a result, large numbers of samples can be rapidly analyzed by high-pressure liquid chromatrography metho...

Journal: :iranian journal of microbiology 0
masoomeh sofian tuberculosis and pediatric infectious research center, arak university of medical sciences, arak, iran. leila safaeipour arak university of medical sciences, arak, iran. arezoo aghakhani clinical research dept., pasteur institute of iran, tehran, iran. mohammad reza sharif department of pediatrics, kashan university of medical sciences, kashan, iran. mohammad banifazl iranian society for support of patients with infectious disease, tehran, iran. alireza sharif department of infectious diseases, kashan university of medical sciences, kashan, iran.

background and objectives: brucellosis is a zoonotic disease and it’s still endemic in iran. there are some reports regarding brucellosis infection in family members sharing same risk factors and remain unrecognized. however , few studies on the importance of family screening are available. we aimed to screen household members of index cases with acute brucellosis for detecting additional unrec...

Journal: :The Biochemical journal 1994
Y S Kuzmenko V N Bochkov M P Philippova V A Tkachuk T J Resink

By use of ligand-blotting techniques, this study investigated lipoprotein-binding proteins in human aortic smooth muscle. PAGE was performed under non-reducing conditions, and, using low-density lipoprotein (LDL) as ligand, with rabbit anti-apolipoprotein (apo) B and 125I-labelled goat anti-rabbit IgG as primary and secondary antibodies respectively, we demonstrate that membranes from human aor...

Journal: :Transfusion 2009
Marco Lai Cinzia Grasso Ilaria Boschi Giuseppe D'Onofrio Vincenzo Pascali Giuseppe Leone

BACKGROUND Anti-D monoclonal antibody (MoAb) reagents have improved D typing in routine tests. However, they exhibit a wide range of reactivity with the weak D phenotype depending on the characteristics of the different MoAbs used. We analyzed the reactivity of immunoglobulin (IgM) anti-D by cluster analysis to characterize MoAb that have similar reactivities with the weak D phenotype. STUDY ...

Journal: :Journal of clinical microbiology 2000
C L Thio K R Nolt J Astemborski D Vlahov K E Nelson D L Thomas

Immunosuppression from human immunodeficiency virus (HIV) may impair antibody formation, and false-negative hepatitis C virus antibody (anti-HCV) tests have been reported in individuals coinfected with HIV and HCV. It is unknown if the frequency of false-negative tests is sufficiently high to change screening recommendations in this setting. Thus, the prevalence of false-negative results for an...

Journal: :Journal of infection in developing countries 2011
Ayman Marei Ghada Boghdadi Nahla Abdel-Hamed Rasha Hessin Theresia Abdoel Henk Smits Fayza Fathey

INTRODUCTION Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-of-care test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. METHODOLOGY Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year p...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد یزد - دانشکده شیمی 1392

in this study, a simple, rapid and selective method was developed for the determination of dexamethasone. the proposed method is based on inhibitory effect of dexamethasone on the oxidation of orange-g by bromate in sulfuric acid media. the reaction was followed spectrophotometrically at 478.5 nm (?max). under optimum experimental conditions, (72.6 ?mol l-1 of orange-g, 0.76 mol l-1 of h2so4, 0...

Jalil Arabkheradmand, Majid Naghdi, Seyed Alireza Mesbah-Namin, Taki Tiraihi,

Background: Bone marrow stromal cells (BMSC) are used as a source for cell therapy in different model for neurological disorder such as stroke and spinal cord injury. However, the transdifferentiation of BMSC into cholinergic phenotype requires more investigation. Methods: BMSC were isolated from adult rats, pre-induced with β-mercaptoethanol (BME) and followed by nerve growth factor (NGF) indu...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه زنجان - دانشکده علوم انسانی 1389

abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...

2005
W. MICHAEL MCDONNELL MICHAEL R. LUCEY

Background. There is a high prevalence of liver disease among the recipients of organs from donors with antibodies to hepatitis C virus (HCV). We undertook a study to determine the frequency of persistent HCV infection, as indicated by the presence of HCV RNA, among both cadaveric organ donors positive for antibodies to HCV (anti-HCV) and the recipients of organs from these donors. Methods. Ser...

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