In diatoms, metabolic activity during long dark periods leads to a chlororespiratory electron flow, which is accompanied by the build-up of a proton gradient strong enough to activate the diadinoxanthin (Ddx) de-epoxidation reaction of the Ddx cycle. In the present study, the impact of chlororespiration on non-photochemical quenching (NPQ) of chlorophyll fluorescence and the regulation of the D...

The main function of the photosynthetic process is to capture solar energy and to store it in the form of chemical 'fuels'. Increasingly, the photosynthetic machinery is being used for the production of biofuels such as bio-ethanol, biodiesel and bio-H2. Fuel production efficiency is directly dependent on the solar photon capture and conversion efficiency of the system. Green algae (e.g. Chlamy...

Inducible gene expression systems are an invaluable tool for studying biological processes. Optogenetic can provide precise control over timing, location, and amplitude using light as the inducing agent. In this protocol, optogenetic system is used to achieve light-inducible in zebrafish embryos. This relies on engineered transcription factor called TAEL based a naturally occurring light-activa...

The quenching of System Ii fluorescence bv System I light was discovered in 196o (ref. I) and was later investigated in detail 2-~. DUYSENS AND SWEEI~S a suggested that System II reduces a compound "Q" , and System I oxidizes this reduced Q back to its oxidized form. The Q in the oxidized, but not in the reduced, form is a quencher of fluorescence. We have measured the time dependence of this q...

Although the traditional Fenton reaction is considered an effective strategy for solving problems caused by environmental pollution, construction of efficient photocatalytic system coordinating challenging. In this study, 2D/2D step-scheme α-Fe2O3/Bi2WO6 (FO/BWO) heterostructure photo-Fenton catalysts were successfully fabricated a facile hydrothermal method. The as-prepared materials character...

The role of cortisol as the only corticosteroid in fish osmoregulation has recently been challenged with the discovery of a mineralocorticoid-like hormone, 11-deoxycorticosterone (DOC), and necessitates new studies of the endocrinology of osmoregulation in fish. Using an in vitro gill explant incubation approach, DOC-mediated regulation of selected osmoregulatory target genes in the gill was in...

Cold acclimation of winter cereals and other winter hardy species is a prerequisite to increase subsequent freezing tolerance. Low temperatures upregulate the expression of C-repeat/dehydration-responsive element binding transcription factors (CBF/DREB1) which in turn induce the expression of COLD-REGULATED (COR) genes. We summarize evidence which indicates that the integration of these interac...

Our laboratory has been working toward increasing our understanding of the genetic control of cold hardiness in blueberry (Vaccinium section Cyanococcus) to ultimately use this information to develop more cold hardy cultivars for the industry. Here, we report using cDNA microarrays to monitor changes in gene expression at multiple times during cold acclimation under Weld and cold room condition...

The induction phenomenon of prompt fluorescence of the photosynthetic system in green plants reflects the adaptation of the electron transport system from a dark adapted state to a steady state under light condition. The appearance of the P-peak in the prompt fluorescence induction curve is explained by the decay of the electrochemical gradient across the thylakoid membrane in the dark. The dec...