The three proteins of the antigen 85 complex (85A, 85B, and 85C), which are major secretory products of Mycobacterium tuberculosis, were purified to homogeneity in large amounts by a combination of chromatography on hydroxylapatite, DEAE-Sepharose, and DEAE-Sephacel and gel filtration from M. tuberculosis culture filtrate. Then we examined the immunological reactivity of the three proteins in t...

AbstractChronic sialadenitis is a disease of salivary glands associated with chronic infections,systemic diseases and silolithiasis pathogenesis of the disease. Primary tuberculoussubmandibulitis is not a reported disease. The diagnosis was confirmed whena 45 years old lady suspicious to submandibular gland tumor was operated on for excisionalbiopsy. Histological examination of the specimen by ...

Mycobacterium tuberculosis infects one third of the world's population. Due to variable efficacy of the Bacille Calmette Guerin (BCG) vaccine, development of novel TB vaccines remains a priority. Here, we demonstrate the protective efficacy of a novel multivalent DNA vaccine, which contains 15 synthetic antigens targeting the Mtb ESX secretion system.

The potentially pathogenic Non-Tuberculosis Mycobacteria (NTM) are emerging nowadays which result in pulmonary and non-pulmonary infections in human. This group of bacteria consists of at least 200 different species. While the pulmonary disease is the most common form of NTM infections, NTM can cause diffused infections as well as extrapulmonary infections in every organ, such as bone marrow, s...

Cases of tuberculosis due to Mycobacterium avium subsp. avium in 52 adult red deer (Cervus elaphus) from a farm were studied using different diagnostic techniques. Immunological probes consisted of the comparative cervical tuberculin (CCT) skin test, the interferon-gamma (IFN-gamma) assay, and 2 enzyme-linked immunosorbent assays (ELISAs) employing either avian purified protein derivatives or p...

background and objectives: tuberculosis (tb) is the oldest known bacterial disease in humans. due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. materials and methods: in this study we used fluorescent amplification–based specific hybridization (flash) pcr to target is6110 for rapid detection of m. tuberculosis (mtb). to investigate the important factors ...

Responsible for 9 million new cases of active disease and nearly 2 million deaths each year, tuberculosis (TB) remains a global health threat of overwhelming dimensions. Mycobacterium bovis BCG, the only licensed vaccine available, fails to confer lifelong protection and to prevent reactivation of latent infection. Although 15 new vaccine candidates are now in clinical trials, an effective vacc...

Although delayed-type hypersensitivity skin testing with tuberculin purified protein derivative (PPD) is the standard for tuberculosis screening, its variability suggests the need for a more sensitive, noninvasive test. An in vitro whole-blood assay has been proposed as an alternative. Using health care worker volunteers, we confirmed the correlation between PPD skin test (PPD-ST) results (posi...