background : despite recent technologic improvements in identifying mycobacterium tuberculosis, we are still facing problems in rapid diagnosis of tuberculosis. the objective of this study is to determine the diagnostic value of a new rapid screening test (patho-tb™) for diagnosis of pulmonary tuberculosis. methods : between september 2006 to august 2007, 178 patients were enrolled in the study...

Background: Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. Objective: The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. Methods: In the present study M. tuberculosis lipid antigens were extracted. Mono...

background: mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. objective: the aim of current investigation was to study the m. tuberculosis lipid antigens in tuberculosis pathogenesis. methods: in the present study m. tuberculosis lipid antigens were extracted. mono...

Mycobacterium tuberculosis genes encoding immunologically relevant proteins were isolated by systematically screening a lambda gt11 recombinant DNA expression library with a collection of murine monoclonal antibodies directed against protein antigens of this pathogen. These antibodies, previously characterized by a World Health Organization workshop on monoclonal antibodies against mycobacteria...

The lambda gt 11 expression vector permitted us to survey protein antigens of Mycobacterium leprae and Mycobacterium tuberculosis expressed in Escherichia coli. Using monoclonal antibodies, recombinant clones were detected producing three major antigens of M. tuberculosis and five major protein antigens of M. leprae. These recombinant antigens produced in E. coli should prove useful for diagnos...

Previous studies of antibody responses to Mycobacterium tuberculosis antigens at different stages of tuberculosis (TB) and in different classes of TB patients provided evidence that the profile of antigens recognized by antibodies is altered with disease progression. M. tuberculosis is a slow-growing organism, and it takes several weeks to months for an infection to progress to clinical TB. App...

Objective(s): Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in ...

b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...

As the disease caused by Mycobacterium tuberculosis continues to be a burden, there is a concerted effort to find new vaccines to combat this problem. One of the important vaccine strategies is whole bacterial vaccines. This approach relies on multiple antigens and built-in adjuvanticity. Other mycobacterial strains which share cross-reactive antigens with M. tuberculosis have been considered a...