نتایج جستجو برای: nano lc retention time
تعداد نتایج: 2012768 فیلتر نتایج به سال:
BACKGROUND Because cerebrospinal fluid (CSF) is in close contact with diseased areas in neurological disorders, it is an important source of material in the search for molecular biomarkers. However, sample handling for CSF collected from patients in a clinical setting might not always be adequate for use in proteomics and metabolomics studies. METHODS We left CSF for 0, 30, and 120 min at roo...
BACKGROUND A large proportion of polar anthropogenic compounds routinely released into the environment comprises homologue series, i.e., sets of chemicals differing in a repeating chemical unit. Using analytical techniques such as liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS), these compounds are readily measurable as signal sets with characteristic differences in...
An overview is given on mass spectrometry-based metabolomics: past, current, and future developments. Understanding the metabolome is challenging due to the enormous diversity of metabolite structures. Metabolomics uses a range of tools, established as service at the NIH West Coast Metabolomics Center at UC Davis. We here explore the use of ion mobility spectrometry (IMS) in combination with li...
Opioid agonists are known to inhibit the activity of locus coeruleus (LC) neurons. In this study, microinjections of the mu-opioid agonist [D-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin (DAMGO; 1.6 microM) bilaterally into the LC caused a significant impairment in the development of a heart-rate (HR) conditioned response (CR). The adverse effect of DAMGO on the HR CR could be reversed with naltrexone ...
Background and purpose: One of the biggest environmental problems of pulp and paper industries is discharge of colored wastewater containing lignin to environment. The aim of this study was to evaluate the TiO2 photocatalytic process Dap with iron in the presence of UV for lignin degradation. Materials and methods: This cross-sectional study was conducted at laboratory scale (in vitro) in a 2-...
Living organisms contain a variety of endogenous peptides that function as significant regulators many biological processes. Endogenous are typically analyzed using liquid chromatography–mass spectrometry (LC-MS). However, due to the low efficiency peptide extraction and abundance in single animal, LC-MS–based peptidomics studies have not facilitated an understanding individual differences tiss...
In spatial comprehensive three-dimensional chromatography (3D-LC) components are separated within a separation space that can lead to unprecedented resolving power, in terms of peak capacity and peak-production rate. The maximum is the product capacities achieved individual dimensions when orthogonal retention mechanisms incorporated. parallel development second- third-dimension stages overcome...
Currently, liquid chromatography-mass spectrometry (LC-MS) is one of the most important analytical technologies for detecting hundreds of metabolites in the field of metabolomics. A recent advance in LC that has impacted metabolomics is the development of UPLC (ultra-performance liquid chromatography). In this chapter, we describe the analytical methodologies for the global metabolic profiling ...
As a part of a project designed to investigate Colchicum species in Jordan, the chemical constituents of Colchicum crocifolium Boiss. (Colchicaceae) were investigated using LC-MS and LC-UV/Vis PDA. A decision tree for working with colchicinods has been developed by incorporating data from LC-UV/PDA and LC-MS. This dereplication strategy draws upon the UV/PDA spectra to classify compounds into o...
Direct LC/MS/MS methods have recently been developed for measuring triphosphate anabolites of several nucleosidic reverse transcriptase inhibitor (NRTI) in peripheral blood mononuclear cells (PBMCs) from HIV-positive patients. Whereas AZT is one of the most-used NRTIs, no such method has been developed for AZT-TP, its active anabolite, mainly because of the presence of endogenous nucleotides th...
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