نتایج جستجو برای: pancreatic islets

تعداد نتایج: 89894  

Journal: :Development 2000
M Gannon M K Ray K Van Zee F Rausa R H Costa C V Wright

We used transgenesis to explore the requirement for downregulation of hepatocyte nuclear factor 6 (HNF6) expression in the assembly, differentiation, and function of pancreatic islets. In vivo, HNF6 expression becomes downregulated in pancreatic endocrine cells at 18. 5 days post coitum (d.p.c.), when definitive islets first begin to organize. We used an islet-specific regulatory element (pdx1(...

Journal: :The Journal of clinical investigation 2013
Nuria C Bramswig Logan J Everett Jonathan Schug Craig Dorrell Chengyang Liu Yanping Luo Philip R Streeter Ali Naji Markus Grompe Klaus H Kaestner

Insulin-secreting β cells and glucagon-secreting α cells maintain physiological blood glucose levels, and their malfunction drives diabetes development. Using ChIP sequencing and RNA sequencing analysis, we determined the epigenetic and transcriptional landscape of human pancreatic α, β, and exocrine cells. We found that, compared with exocrine and β cells, differentiated α cells exhibited many...

2013
Behzad Mobini

The objective of this investigation was to study the histochemical and histological structure of the pancreas in pigeons. Samples were obtained from 9 male and 9 female adult clinically healthy pigeons. Tissue sections were stained with haematoxylin eosin, Verhoeff’s, Gomori’s method for reticulum, Masson’s trichrome and Maldonado’s methods for pancreatic islet cells. The pancreas of adult pige...

2009
Ludmila Prokunina-Olsson Cullan Welch Ola Hansson Neeta Adhikari Laura J. Scott Nicolle Usher Maurine Tong Andrew Sprau Amy Swift Lori L. Bonnycastle Michael R. Erdos Zhi He Richa Saxena Brennan Harmon Olga Kotova Eric P. Hoffman David Altshuler Leif Groop Michael Boehnke Francis S. Collins Jennifer L. Hall

Common variants in the transcription factor 7-like 2 (TCF7L2) gene have been identified as the strongest genetic risk factors for type 2 diabetes (T2D). However, the mechanisms by which these non-coding variants increase risk for T2D are not well-established. We used 13 expression assays to survey mRNA expression of multiple TCF7L2 splicing forms in up to 380 samples from eight types of human t...

Journal: :The Journal of biological chemistry 2014
Preetha Shridas Lubna Zahoor Kathy J Forrest Joseph D Layne Nancy R Webb

Group X secretory phospholipase A2 (GX sPLA2) potently hydrolyzes membrane phospholipids to release arachidonic acid (AA). While AA is an activator of glucose-stimulated insulin secretion (GSIS), its metabolite prostaglandin E2 (PGE2) is a known inhibitor. In this study, we determined that GX sPLA2 is expressed in insulin-producing cells of mouse pancreatic islets and investigated its role in b...

Journal: :Diabetes 2002
Werner Gurr Reza Yavari Li Wen Margaret Shaw Conchi Mora Laurence Christa Robert S Sherwin

Genes overexpressed in pancreatic islets of patients with new-onset type 1 diabetes are potential candidates for novel disease-related autoantigens. RT-PCR-based subtractive hybridization was used on islets from a patient who died at the onset of type 1 diabetes, and it identified a type 1 diabetes-related cDNA encoding hepatocarcinoma-intestine-pancreas/pancreatic-associated protein (HIP/PAP)....

2015
Peter Buchwald Sirlene R Cechin Jessica D Weaver Cherie L Stabler

BACKGROUND In type 1 diabetic patients, who have lost their ability to produce insulin, transplantation of pancreatic islet cells can normalize metabolic control in a manner that is not achievable with exogenous insulin. To be successful, this procedure has to address the problems caused by the immune and autoimmune responses to the graft. Islet encapsulation using various techniques and materi...

Journal: :Clinical and Developmental Immunology 2003
Jan-Luuk Hillebrands Nienke van der Werf Flip A. Klatter Cathrien A. Bruggeman Jan Rozing

BACKGROUND As one of the natural perturbants, infection with cytomegalovirus (CMV) is believed to play a role in the development of Type I diabetes. Using the DP-BB rat model for autoimmune diabetes, we here report about possible mechanisms responsible for R(at)CMV-induced accelerated onset of diabetes. METHODS Rats were i.p. infected with 2 x 10(6) plaque forming units (pfu) RCMV and followe...

2017
H. Napierala K.-H. Hillebrandt N. Haep P. Tang M. Tintemann J. Gassner M. Noesser H. Everwien N. Seiffert M. Kluge E. Teegen D. Polenz S. Lippert D. Geisel A. Reutzel Selke N. Raschzok A. Andreou J. Pratschke I. M. Sauer B. Struecker

Decellularization of pancreata and repopulation of these non-immunogenic matrices with islets and endothelial cells could provide transplantable, endocrine Neo- Pancreata. In this study, rat pancreata were perfusion decellularized and repopulated with intact islets, comparing three perfusion routes (Artery, Portal Vein, Pancreatic Duct). Decellularization effectively removed all cellular compon...

Journal: :General physiology and biophysics 2005
Z Bacová M Najvirtová O Krizanová S Hudecová S Zórad V Strbák J Benický

Neonatal STZ (nSTZ) treatment results in damage of pancreatic B-cells and in parallel depletion of insulin and TRH in the rat pancreas. The injury of B-cells is followed by spontaneous regeneration but dysregulation of the insulin response to glucose persists for the rest of life. Similar disturbance in insulin secretion was observed in mice with targeted TRH gene disruption. The aim of present...

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