It is generally accepted that the behaviourally stimulant properties of D-amphetamine can be at­ tributed to its ability to release catecholamines from intraneuronal storage sites and block their re­ uptake lf 2. Since lipids constitute an essential part of cellular membranes 3, and there is some evidence that they may be involved in ion transport pro­ cesses in nerve membranes 4, it seems poss...

The glycosylphosphatidylinositol (GPI)-anchored proteins are widely distributed in eukaryotic cells, from yeasts to mammals. A number of proteins, such as glycosyltransferases, are necessary for GPI anchor biosynthesis. Cloning of genes encoding these proteins is required for analyses of their nature and the biosynthetic pathway. Here we report a new method of expression cloning that is applica...

Many water-soluble compounds have been shown to pass from the small intestinal mucosa into the lumen. In this work, the loss of lipids from the mucosa was investigated by perfusion experiments in rats, using 0-15M NaCl or buffer solutions over range of pH, with or without the addition of 5-7 or 11-4mM taurocholic acid. Perfusates were extracted for the estimation of individual lipids and for DN...

Non-alcoholic fatty liver disease (NAFLD) is clinically characterized as uncontrolled fat accumulation in the liver resulting in inflammatory responses, insulin resistance, elevated liver enzymes, and ultimately liver failure. Currently, there are no clinical lipid markers detectable by blood test to ascertain the manifestation of improper fat regulation in the liver. The cell membranes of hepa...

The apparent turnovers of mitochondrial DNA and of the phospholipids, cardiolipln, lecithin, and phosphatidyl ethanolamine, have been determined simultaneously in the mitochondria of rat liver, kidney, brain, and heart. The turnover rate of mitochondrial DNA in liver was similar to previously reported turnover rates of other mitochondrial components. Mitochondrial DNA has a half-life of 6.7 day...

Major depressive disorders (MDDs) are often associated with a deficiency in long-chain omega-3 polyunsaturated fatty acids (?-3 PUFAs), as well signs of low-grade inflammation. Epidemiological and dietary studies suggest that high intake fish, the major source ?-3 PUFAs, is lower rates MDDs. Meta-analyses randomized placebo-controlled PUFAs intervention-trials primarily eicosapentaenoic acid (E...