A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

A feasibility study with three pre-conditioning times was carried out to examine the effect of anaerobic pre-treatment on phosphorus removal by aeration. The results showed that soluble phosphorus could be decreased from 120 mg L−1 to about 40 mg L−1 within one-day aeration. However, soluble phosphorus concentration was found not affected by pre-conditioning, but more pH dependent. Anaerobic pr...

A two-stage thermal desorption/secondary electrospray ionisation/time-of-flight mass spectrometry for faster targeted breath profiling has been studied. A new secondary electrospray ionisation (SESI) source was devised to constrain the thermal desorption plume and promote efficient mixing in the ionization region. Further a chromatographic pre-separation stage was introduced to suppress interfe...

A two-stage thermal desorption/secondary electrospray ionization/time-of-flight mass spectrometry for faster targeted breath profiling has been studied. A new secondary electrospray ionization (SESI) source was devised to constrain the thermal desorption plume and promote efficient mixing in the ionization region. Further, a chromatographic pre-separation stage was introduced to suppress interf...

A method allowing initial sequencing yields of 60-85% to be consistently obtained from samples prepared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretic transfer is described in detail. Conducting electrophoresis at a pH near neutrality is the single most important of the modifications made to earlier procedures, but pre-electrophoresis in the presence of glutath...

Independent Component Analysis (ICA) is a classical method for Blind Source Separation (BSS). In this paper, we are interested in ICA in the presence of noise, i.e., the noisy ICA problem. Pseudo-Euclidean Gradient Iteration (PEGI) is a recent cumulant-based method that defines a pseudo Euclidean inner product to replace a quasi-whitening step in Gaussian noise invariant ICA. However, PEGI has ...

We consider blind source separation in chemical analysis focussing on the 3D fluorescence spectroscopy framework. We present an alternative method to process the Fluorescence Excitation-Emission Matrices (FEEM): first, a preprocessing is applied to eliminate the Raman and Rayleigh scattering peaks that clutter the FEEM. To improve its robustness versus possible improper settings, we suggest to ...

26 27 28 29 UN CO RR EC TE D Abstract. Current methods for the localization of EEG and event-related potentials (ERP) sources assume that sources are either discrete (dipole-like) or distributed. While both types of sources are likely to contribute significantly to EEG and ERP signals, each method adopts only one of these models and thus may localize the sources of other type incorrectly or not...

Independent component analysis (ICA) is an unsupervised technique for blind source separation, and the ICA algorithms using nongaussianity as the measure of mutual independence have been also used for projection pursuit or visualization of multivariate data for knowledge discovery in databases (KDD). However, in real applications, it is often the case that we fail to extract useful latent varia...