detection of bovine leukemia virus antigens can induces humoral immunity in cow were evaluated. fifteen lymph node from infected cows that had positive results in agid and elisa tests for blv and have clinical signs of lymphosarcoma, in addition five lymph node from apparently healthy cows that had negative results in serological tests. after preparation of extract of lymph nodes, all of the sa...

A panel of seven recombinant antigens, derived from Ehrlichia phagocytophila (the agent of human granulocytic ehrlichiosis), was evaluated by class-specific enzyme-linked immunosorbent assays (ELISAs) for utility in the diagnosis of the infection. Fourteen genomic fragments, obtained by serologic expression screening, contained open reading frames (ORFs) encoding 16 immunodominant antigens. Ele...

We have studied the antibodies to sexual stage antigens of Plasmodium falciparum in human sera from Papua New Guinea where intense transmission of P. falciparum occurs as well as the less prevalent P. malariae and P. vivax. In extracts of gametes of P. falciparum we have studied the reactivity of serum antibodies with antigens labeled with 125I on the surface of the gametes as well as intracell...

conclusions the canola oilseeds could provide a useful expression system to produce hcv core protein as a vaccine candidate. results western blot analysis using anti-his antibody confirmed the presence of a 15 kda protein in the seeds of t1 transgenic lines. the amount of antigenic protein accumulated in the seeds of these transgenic lines was up to 0.05% of the total soluble protein (tsp). mat...

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

Capsular polysaccharides are the primary antigenic components involved in protective immunity against encapsulated bacterial pathogens. Although immunization of adolescents and adults with polysaccharide antigens has reduced pathogen disease burden, pure polysaccharide vaccines have proved ineffective at conferring protective immunity to infants and the elderly, age cohorts that are deficient i...

Autoimmune responses against posttranslationally modified antigens are a hallmark of several autoimmune diseases. For example, antibodies against citrullinated protein antigens (ACPA) have shown their relevance for the prognosis and diagnosis of rheumatoid arthritis (RA), and have been implicated in disease pathogenesis. It is conceivable that other autoantibody systems, recognizing other postt...

Dietary antigens are normally rendered nonimmunogenic through a poorly understood "oral tolerance" mechanism that involves immunosuppressive regulatory T (Treg) cells, especially Treg cells induced from conventional T cells in the periphery (pTreg cells). Although orally introducing nominal protein antigens is known to induce such pTreg cells, whether a typical diet induces a population of pTre...

AIM To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM). METHODS The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of a...