Many biological processes are regulated through dynamic protein phosphorylation. Monitoring disease-relevant phosphorylation events in circulating biofluids is highly appealing but also technically challenging. We introduce here a functionally tunable material and strategy, extracellular vesicles to phosphoproteins (EVTOP), which achieves one-pot (EVs) isolation, extraction, digestion of EV pro...

This study aimed to examine whether a single ingestion of arginine activates the mammalian target rapamycin complex 1 (mTORC1) in rat fast- and slow-twitch skeletal muscles. In first experiment, rats were orally administered (3 or 10 mmol/kg body weight) water. The plantaris, gastrocnemius, soleus muscles excised h after administration. Immunoblot analysis showed that administration with higher...

The role of tyrosine-specific phosphorylation in v-fms-mediated transformation was examined by immunoblotting techniques together with a high-affinity antibody that is specific for phosphotyrosine. This antiphosphotyrosine antibody detected phosphorylated tyrosine residues on the gp140v-fms molecule, but not gP180v-fms or gp120v-fms, in v-fms-transformed cells. This antibody also identified a n...

In this study, the effect of topiramate, as an antiepileptic drug, was evaluated on morphine craving in rats. The conditioned place preference (CPP) test was used for this purpose. Repeated administration of morphine (10 mg/kg, i.p. for 4 days) induced significant CPP. Administration of topiramate (50 and 100 mg/kg, i.p. for 4 days) with each morphine administration decreased the acquisition of...

The phosphorylation of specific membrane proteins by endogenous protein kinase in synaptic membrane fractions from rat cerebrum has been studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The endogenous phosphorylation of two specific membrane proteins was found to be regulated by adenosine 3’ : 5’-monophosphate (cyclic AMP). The minimal mol...

ABSTRACT First Person is a series of interviews with the first authors selection papers published in Journal Cell Science, helping early-career researchers promote themselves alongside their papers. Joanna Wenda author on ‘ Mitotic chromosome condensation requires phosphorylation centromeric protein KNL-2 C. elegans’, JCS. PhD student (in process graduating) lab Florian Steiner at Department Mo...

Significance Lignin deposition in plants is affected by environmental stress, and stress-signaling involves increases the levels of plant hormone abscisic acid (ABA). Here we show, using a combination biochemical genetic approaches, how ABA can regulate lignin biosynthesis. This phosphorylation master transcription factor NST1 family protein kinases (SnRK2s) that are themselves activated as res...