The enzyme lysozyme served as a prototype to study the early stages of protein crystallization or precipitation. The crystallization and precipitation aggregates reactions were initiated by supersaturating the lysozyme solutions through addition of NaCI and (NH4)2S04 respectively and monitored via photon correlation spectroscopy. Laplace inversion of the spectra revealed in general bimodal part...

We evaluated three methods for isolating ferritin for use as a standard, with respect to purity of the products, ease of preparation, and yield. Examination of the respective products by gel filitration on Sephadex G-200 and Sepharose 6B suggested that the preparations isolated by ammonium sulfate and cadmium sulfate precipitation (Method 1) and by ultracentrifugation (Method 2) were homogeneou...

objective: helicobacter pylori is a spiral, microaerophilic gram negative bacterium, that multiplies and causes infection in human gastric mucosal layer. h.pylori infection, followed by destruction of gastric epithelial tissue, leads to gastric chronic inflammation, which can cause gastric and peptic ulcers. new approaches have focused on using specific treatments, such as immunotherapy, to era...

In this paper we present a synthesis method for obtaining hydroxyapatite nanopowders by chemical precipitation. As the starting reagents, analytical grade Ca(OH)2, H3PO4 and NH4OH were used. . In order to study the factors that have an important influence on the chemical precipitation process a experimental platform has been designed for hydroxyapatite synthesis. The experimental platform was d...

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BMCL26 is a potential drug derived from nimesulide, which has exhibited the substantial anti-parasitic activity in various cell lines. To conduct various pharmacological and toxicological properties of this drug, we developed and validated a rapid LC-MS/MS method for its quantification in accordance with the FDA guidelines. Protein precipitation with 0.1% formic acid in acetonitrile was used to...

Recent studies have shown that sea kraits (Laticauda spp.)--amphibious sea snakes--dehydrate without a source of fresh water, drink only fresh water or very dilute brackish water, and have a spatial distribution of abundance that correlates with freshwater sites in Taiwan. The spatial distribution correlates with sites where there is a source of fresh water in addition to local precipitation. H...