Individual genetic background together with environmental effects are thought to be behind many human complex diseases. A number of genetic variants, mainly single nucleotide polymorphisms (SNPs), have been shown to be associated with various pathological and inflammatory conditions, representing potential therapeutic targets. Prostaglandins (PTGs) and leukotrienes (LTs) are eicosanoids derived...

RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA. RNAi is mediated by 21-23 nucleotide small interfering RNAs (siRNAs) which are produced from long double-stranded RNAs by RNAse II-like enzyme Dicer. The resulting siRNAs are incorporated into a RNA-induced silencing complex (RISC) that targets and cleaves mRNA complementary to the siRNAs. Since its ...

background: rna interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. rorc2 is the key transcription factor orchestrating th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. the aim of this study was to design efficient sirnas specific for rorc2 and to evaluate different criteria...

relieve the inhibition, suggesting micrococcal nuclease When double-stranded RNA (dsRNA) corresponding to was not merely competing for nucleic acid binding.) a sense and antisense sequence of an endogenous Further pieces of the puzzle are provided by beautiful mRNA is introduced into a cell, in organisms ranging work from a collaborative effort of Phil Zamore, Tom from trypanosomes to mice, the...

Engineered peptide based biocompatible, siRNA transporter can transfect primary and TNBC cells with efficient long-term gene silencing. Nanocomplex silencing Notch1 exhibits synergistic interaction metformin amplifies anticancer therapy.

UNLABELLED Palm oil obtained from E. guineensis Jacq. Tenera is known to have about 44% of palmitic acid (C16:0). Palmitoyl-Acyl Carrier Protein Thioesterase (PATE) is one of the key enzymes involved in plastidial fatty acid biosynthesis; and it determines the level of the C16:0 assimilation in oilseeds. This enzyme's activity in oil palm is responsible for high (> 44 % in E. guineensis Jacq. T...

Predicting unanticipated harmful effects of chemicals and drug molecules is a difficult and costly task. Here we utilize a 'big data compacting and data fusion'-concept to capture diverse adverse outcomes on cellular and organismal levels. The approach generates from transcriptomics data set a 'predictive toxicogenomics space' (PTGS) tool composed of 1,331 genes distributed over 14 overlapping ...

RNA silencing is a biochemical mechanism that regulates gene expression by post-transcriptionally activating a sequence-specific RNA degradation by three different pathways: (i) small interfering RNA (siRNA) silencing of exogenous mRNA; (ii) micro RNA (miRNA) silencing of endogenous mRNAs, and (iii) associated with DNA methylation and suppression of transcription [2]. These processes share thre...

In time, we have become gradually more aware of the fact that RNAs play a vital role in regulation of gene expression. The discovery of RNA interference (RNAi) also called post transcriptional gene silencing (PTGS), gave a booster shot to the world of RNA and molecular biology, a set of reactions involving the sequence-specific degradation of targeted mRNA by short double-stranded RNA molecules...

Following the design demands of protective switch used in ±800 kV hybrid UHVDC system, a plasma-triggered-based gas (PTGS) is developed by performing comparative study its dynamic performance considering arc behaviour induced transverse magnetic field (TMF)-based electrode PTGS. The PTGS-based electrode, as core component, designed with helical-slotted structure, TMF could drive between moving ...