نتایج جستجو برای: ptgs gene silencing

تعداد نتایج: 1153734  

ژورنال: :بیماریهای گیاهی 0
جعفر نیکان نویسنده

ژن خاموشی پس از ترا نویسی (post-transcriptional gene silencing =ptgs) به عنوان یک مکانیسم طبیعی که به وسیله آن گیاهان اسیدهای نوکلئیک بیگانه مانند ژنوم ویروس ها را شناسایی و تجزیه می کنند مطرح است. در این مطالعه فرض شد که در مقاومت سیب زمینی  همسانه g8107(1)  در برابر تجمع plrv نیز چنین مکانیسمی دخالت دارد. این فرضیه با آزمایش انتقال ویروس از طریق پیوند آزمون شد. در این آزمایش قطعاتی از ساقه ها...

ژورنال: :بیماریهای گیاهی 2014
جعفر نیکان

ژن خاموشی پس از ترا نویسی (post-transcriptional gene silencing =ptgs) به عنوان یک مکانیسم طبیعی که به وسیله آن گیاهان اسیدهای نوکلئیک بیگانه مانند ژنوم ویروس ها را شناسایی و تجزیه می­کنند مطرح است. در این مطالعه فرض شد که در مقاومت سیب زمینی  همسانه g8107(1)  در برابر تجمع plrv نیز چنین مکانیسمی دخالت دارد. این فرضیه با آزمایش انتقال ویروس از طریق پیوند آزمون شد. در این آزمایش قطعاتی از ساقه­ها...

Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...

Journal: :research in molecular medicine 0
maryam zangi iranian research organization for science and technology (irost) sh. ehsani rad st., enqelab st., parsa sq., ahmadabad mostoufi rd., azadegan highway, p. o. box 3353-5111, tehran 3353136846, i. r. iran tel: (+9821)56276031-2 (+9821)56276026 p. o. box 3353 hamideh ofoghi iranian research organization for science and technology (irost) sh. ehsani rad st., enqelab st., parsa sq., ahmadabad mostoufi rd., azadegan highway, p. o. box 3353-5111, tehran 3353136846, i. r. iran tel: (+9821)56276031-2 (+9821)56276026 p. o. box 3353 zahra amini-bayat iranian research organization for science and technology (irost) sh. ehsani rad st., enqelab st., parsa sq., ahmadabad mostoufi rd., azadegan highway, p. o. box 3353-5111, tehran 3353136846, i. r. iran tel: (+9821)56276031-2 (+9821)56276026 p. o. box 3353 parastoo ehsani molecular biology dept. pasteur institute of iran, tehran, iran.

background: the potential of plants as a safe and eukaryotic systems, are considered for producing recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (ptgs) response in this new technology. the use of viral suppressors of gene silencing can prevent ptgs and improve transient expression level of for...

Journal: :Current Biology 2001
Titia Sijen Irma Vijn Alexandra Rebocho Rik van Blokland Dick Roelofs Joseph N.M. Mol Jan M. Kooter

Two distinct gene-silencing phenomena are observed in plants: transcriptional gene silencing (TGS), which involves decreased RNA synthesis because of promoter methylation, and posttranscriptional gene silencing (PTGS), which involves sequence-specific RNA degradation. PTGS is induced by deliberate [1-4] or fortuitous production (R.v.B., unpublished data) of double-stranded RNA (dsRNA). TGS coul...

Abdul Qayyum Rao, Farooq Ihsan, Idrees Ahmad Nasir Muhammad Rafiq, Sajed Ali, Shahid Javed Butt Tayyab Husnain,

Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

2012
Vincent Jauvion Maud Rivard Nathalie Bouteiller Taline Elmayan Hervé Vaucheret

BACKGROUND RNA-DEPENDENT RNA POLYMERASE6 (RDR6) and SUPPRESSOR of GENE SILENCING 3 (SGS3) are required for DNA methylation and post-transcriptional gene silencing (PTGS) mediated by 21-nt siRNAs produced by sense transgenes (S-PTGS). In contrast, RDR2, but not RDR6, is required for DNA methylation and TGS mediated by 24-nt siRNAs, and for cell-to-cell spreading of IR-PTGS mediated by 21-nt siRN...

Journal: :The EMBO journal 1998
G Brigneti O Voinnet W X Li L H Ji S W Ding D C Baulcombe

Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of ...

Journal: :Trends in genetics : TIG 2001
H Vaucheret M Fagard

Gene silencing can occur either through repression of transcription, termed transcriptional gene silencing (TGS), or through mRNA degradation, termed post-transcriptional gene silencing (PTGS). Initially, TGS was associated with the regulation of transposons through DNA methylation in the nucleus, whereas PTGS was shown to regulate virus infection through double-stranded RNA in the cytoplasm. H...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1999
O Voinnet Y M Pinto D C Baulcombe

In transgenic and nontransgenic plants, viruses are both initiators and targets of a defense mechanism that is similar to posttranscriptional gene silencing (PTGS). Recently, it was found that potyviruses and cucumoviruses encode pathogenicity determinants that suppress this defense mechanism. Here, we test diverse virus types for the ability to suppress PTGS. Nicotiana benthamiana exhibiting P...

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