background and aims. nano-ionomer (ni) interacts with tooth structures superficially, and there is a concern about the enamel bonding ability of mild self-etch ketac primer. this study compared the effect of different adhesive procedures (self-etching and etch-and-rinse approach) on long-term marginal microleakage of nano-filled resin-modified glass-ionomer (ni) cervical restorations. materials...

The purpose of this study was to evaluate the antibacterial activity of a modified self-etching primer incorporating chitosan and whether this modification affected the bond strength to radicular dentin. A modified self-etching primer was prepared by adding chitosan solutions at 0.03%, 0.06%, 0.12% and 0.25% (W/W) to RealSeal selfe-tching primer. RealSeal primer without chitosan was used as the...

Prediction programs for general PCR (or random PCR) products had been developed [3, 2]. These programs had been experimentally shown to be highly accurate in its prediction [2]. On the other hand, random PCR have been demonstrating that it is very useful as an elementary technology of genome profiling [1], in which random PCR products (a set of DNA fragments extracted from various parts of geno...

Penelitian ini bertujuan untuk mengetahui dan menilai bagaimana peran Media Sosial E-Commerce bagi UMKM dalam meningkatkan Volume Penjualan. Objek dari penelitian adalah para pelaku yang ada di Kecamatan Belopa. Metode digunakan survei kuesioner, sampel sebanyak 100 responden, pengambilan dilakukan secara acak (random sampling) menggunakan teknik sampling sederhana (simple random sampling). Kue...

Next-generation sequencing (NGS) is a powerful tool for analyzing environmental DNA and provides the comprehensive molecular view of microbial communities. For obtaining the copy number of particular sequences in the NGS library, however, additional quantitative analysis as quantitative PCR (qPCR) or digital PCR (dPCR) is required. Furthermore, number of sequences in a sequence library does not...

genetics Primer g enetics is the study of genes— the heritable information that contains the codes for proteins and other molecules which form and maintain an organism's structure and function. In most organisms, these genes are found in strands of deoxyribonucleic acid (DNA) molecules. The specific structure of the DNA (described below) ensures that the genetic information can be passed from o...

The significance of the precise position of the hydroxyl at the 3'-end of an RNA primer for nonenzymatic template-directed primer extension is not well understood. We show that an RNA primer terminating in 3'-hydroxymethyl-2',3'-dideoxy-guanosine has greatly diminished activity, suggesting that the spatial preorganization of the terminal sugar contributes significantly to the efficiency of prim...

Introduction: Nowadays, the main focus of dental studies is on adhesive dental materials; since clinical long-term success of bonded restorations depended more on marginal microleakage minimization. So, the aim of this study was Evaluation of Diode laser irradiation effect on microleakage in class V composite restoration before and after adhesive application. Materials and methods: In this in v...