نتایج جستجو برای: rdna
تعداد نتایج: 11414 فیلتر نتایج به سال:
BACKGROUND Ribosomal deficits are documented in mild cognitive impairment (MCI), which often represents an early stage Alzheimer's disease (AD), as well as in advanced AD. The nucleolar rRNA genes (rDNA), transcription of which is critical for ribosomal biogenesis, are regulated by epigenetic silencing including promoter CpG methylation. METHODOLOGY/PRINCIPAL FINDINGS To assess whether CpG me...
The nuclear ribosomal repeats for the 18S, 5.8S, and 26S RNAs of two closely related Picea (spruce) species were characterized by restriction mapping and Southern blot hybridization. Restriction polymorphisms were identified in the IGS and ITS sequences; however, no polymorphism was species specific. As many as five different rDNA repeat units were observed in individual genomes. The repeat siz...
We report sensitive radioimmunoassay procedures for glucagon-like immunoreactivity and for pancreatic glucagon, with use of antisera generated in rabbits by the injection of glucagon covalently coupled to thyroglobulin. Optimum assay conditions were determined. These assays have absolute limits of sensitivity of 10 to 20 pg, an intra-assay coefficient of variation of 8%, and an inter-assay coef...
The hyper-variable V4 and V9 regions of the small subunit (SSU) rDNA have been targeted for assessing environmental diversity of microbial eukaryotes using next generation sequencing technologies. Here, we explore how the genetic distances among these short fragments compare with the distances obtained from near full-length SSU-rDNA sequences by comparing all pairwise estimates, as well as with...
Ribosomal RNAs (rRNAs) are encoded by multicopy families of identical genes. In Dictyostelium and other protists, the rDNA is carried on extrachromosomal palindromic elements that comprise up to 20% of the nuclear DNA. We present the sequence of the 88 kb Dictyostelium rDNA element, noting that the rRNA genes are likely to be the only transcribed regions. By interrogating a library of ordered Y...
Treatment of chronic prostatitis/chronic pelvic pain syndrome is often empirical because clinical culture methods fail to detect prostate-associated pathogens in >90% of patients. Previously, we tested a variety of specific-microorganism PCRs and began a DNA sequence study after we found that 77% of prostatitis patients were PCR positive for prokaryotic rRNA-encoding DNA sequences (rDNAs) despi...
در مطالعه فونستیک نماتودهای روده ای (brachirusorientalis(bloch & schneider 1801 از سواحل خوزستان در خلیج فارس، گونه ی جدیدی از خانواده ی anisakidae، متعلق به جنس ward & magath, 1917hysterothylaciumتوصیفشد. hysterothylacium sp. n. با داشتن سکوم روده ای کوتاه (15-44/12 درصد طول مری) و ونتریکولار اپندیکس تقریبا بلند (با نسبت طول سکوم به اپندیکس26/0-23/0)، با دارا بودن آرایش ساختارهای کوتیکولی دم د...
Eight populations of Sitka spruce (Picea sitchensis (Bong.) Carr.) and interior spruce (Picea glauca (Moench) Voss x Picea engelmannii Parry ex. Engelm.) seedlings were sampled from a zone of Sitka-interior spruce introgression in British Columbia, Canada. Restriction fragment length polymorphisms of the nuclear ribosomal RNA genes (rDNA) were used to define species-specific hybridization patte...
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