The objective of this study is to compare and assess the effectiveness real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), selective agar plate method for detection Salmonella spp. Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, performance three methods (RT-PCR [SureTectTM kit PowerChekTM kit], LAMP [3M MDS], agar) were similar at 0-10...

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

background: hepatitis g virus (gbv-c) is a single strand rnapositive virus and is a member of flaviviridae family. infection with the virus is prevalent add has worldwide distribution. accurate diagnosis of the virus is very important, because of co-infection with other important viruses like hcv, hbv and hiv, it may influence on pathogenesis, disease progression and response to viral therapy. ...

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

Birds play a central role in WNV epidemiology by spreading and amplifying the virus. Increasing numbers of WNV isolates are detected in Europe, and the virulence of these genetically variable isolates is not well characterized for birds. Therefore, we investigated whether SPF chickens could be a valuable avian model for the pathotyping of WNV strains. One-day-old SPF chickens were inoculated su...

Real-time RT-PCR is used as a gold standard method for the diagnosis of COVID-19. Since real-time RT PCR nucleic acid-based, it highly sensitive and specific test. However, this test takes 4–8 h to generate results and, in emergency settings, delay may prove fatal certain patients. The frequent surge COVID cases increases patient load settings. Thus, acid-based rapid POC required that can quick...