Experiments were carried out in vivo to investigate the pathways of ammonia incorporation into rumen bacteria, bacterial fractions and free amino acids within the bacteria. Steers were alternately given two isoenergetic, isonitrogenous diets containing the nitrogen mainly as either urea or decorticated groundnut meal (DCGM). At the end of each period on a given diet, a solution of 15NH4Cl was i...

10 healthy Indian Jersey/Red Sindhi crossbred nonpregnant cows were subjected to transabdominal ultrasonography to develop baseline topographical data of the rumen. The wall of the rumen could be identified as a thick echogenic line adjacent to the left abdominal wall from left flank to 8th intercostal space. The motility pattern of rumen was characterized by approximately 1 contraction every m...

I. 15NH4C1 was continuously infused for periods of 120-216 h into the rumens of sheep which were allowed to feed 2 out of every 10 min. These treatments achieved steady metabolic states and allowed the assessment of nitrogen conversions by means of tracer methodology. The sheep were given either a barley diet or one of three hay diets. In two trials, the flow of abomasal material was determined...