ABSTRACT Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure the microorganism. current study aimed optimize iELISA from total cell antigen, applying it bovine serum samples, and evaluate risk factors. Serum samples were o...

Phenoloxidase (Po) activity was measusred spectrophotometrically in serum, plasma and haemocytes (HLS) of the freshwater crayfish (Pontastacus leptodactylus). The highest activity was found in HLS suggesting that the haemocytes are the major source of the Po or its proform prophenoloxidase (proPO) in crayfish. Furthermore, the enzyme activity in serum samples was reduced after freezing the samp...

The aim of the study was to evaluate level amylase in serum diabetic patients compared healthy controls, glucose, lipids, lipoproteins, thyroid hormones, electrolytes (calcium, potassium, and sodium) transporter enzymes were also studied. (AST), (ALT),(ALP). enzyme purified association between previously mentioned variants it studied other biochemical variables found that there a significant po...

Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization bulky devices costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free sensing method with dual-stage signal amplification ability based coupling use catalytic ...

A very rapid method of agar gel electrophoresis on glass slides, together with a superior visualization technique employing simultaneous coupling of a hydrolysed naphthol substrate, have been developed for the identification of the tissues of origin of serum alkaline phosphatase. Combined with L-phenylalanine inhibition, specific for the intestinal enzyme, and heat inactivation, specific for th...

A rapid and accurate enzymatic assay has been developed for the determination of blood serum levels of BB-K8 and other suitable aminoglycoside antibiotics.