Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had been treated with PEG and in phagosomes of CHW-1102 in cultures not treated w...

OBJECTIVE To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity. DESIGN Prospective, comparative, in vitro study. SETTING Andrology laboratory at tertiary care hospital. PATIENT(S) Thirteen normozoospermic donors. INTERVENTION(S) Semen samples from 13 subjects were incubated in human tubal fluid media (HT...

BACKGROUND Evaluating the significance and the effects of plant-derived drugs on laboratory animal's fertility was recognized. There was antioxidant activity reported from Heracleum persicum (Golpar). OBJECTIVE Current study aims to study the antioxidant effect of Golpar extracts on sperm parameters and chromatin quality in mice. MATERIALS AND METHODS Eighteen adult male mice were divided t...

Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile donors comparing the sperm chromatin structure assay (SCSA), TdT-mediated-dUTP nick end labeling (TUNEL), the sperm chromatin dispersion (SCD) test, and acridine orange staining technique (AOT). The TUNEL...

In most animals, the extreme compaction of sperm DNA is achieved after the massive replacement of histones with sperm nuclear basic proteins (SNBPs), such as protamines. In some species, the ultracompact sperm chromatin is stabilized by a network of disulfide bonds connecting cysteine residues present in SNBPs. Studies in mammals have established that the reduction of these disulfide crosslinks...

BACKGROUND Linker histone H1 has been studied in vivo and using reconstituted chromatin, but there have been few systematic studies of the effects of the cellular environment on its function. Due to the presence of many other chromatin factors and specific chaperones such as RanBP7/importin beta that regulate histone H1, linker histones likely function differently in vivo than in purified syste...

background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

Background: Oxidative stress occurs when the oxidative homeostasis is damage. Excessive ROS are generated and this reaction has been proposed to produce marked damage to the structure and function of sperm. Materials and Methods: This study was designed to investigate the protective effects of of American Ginseng against cyclophosphamide - induced damage in the rat sperm. For this experiment, t...