Protein-protein interactions are essential for maintaining cell structure and for executing almost all cellular processes. Determination of where and how each protein interacts with its partners provides significant insight into proteins' cellular roles. Although several assays, such as FRET and bimolecular fluorescence complementation (BiFC), have been developed and widely used for visualizati...

The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter. With an anti-HBV pri-miR31 mimic integrated between two HBV-specific gRNAs, both gRNAs could be separated from the long transcript of gRNA-miR-HBV-gRNA ternary cassette thro...

Density, viscosity and refractive index of pure, binary and ternary mixtures of diethanolamine (DEA) and methyl diethanolamine (MDEA) with water have been measured at different temperatures from 288.15 K to 323.15 K at atmospheric pressure. Also, this study presents the effects of mixture composition and temperature upon these thermo- physical properties. To measure the density and refractive i...

We developed a novel small interfering RNA (siRNA) delivery system using a ternary complex with polyethyleneimine (PEI) and γ-polyglutamic acid (γ-PGA), which showed silencing effect and no cytotoxicity. The binary complexes of siRNA with PEI were approximately 73-102 nm in particle size and 45-52 mV in ζ-potential. The silencing effect of siRNA/PEI complexes increased with an increase of PEI, ...

The tyrosine kinase c-Src is upregulated in numerous human cancers, implying a role for c-Src in cancer progression. Previously, we have shown that sequestration of activated c-Src into lipid rafts via a transmembrane adaptor, Cbp/PAG1, efficiently suppresses c-Src-induced cell transformation in Csk-deficient cells, suggesting that the transforming activity of c-Src is spatially regulated via C...

A static scission configuration in cold ternary fission has been considered in the framework of mean field approach. The inverse scattering method is applied to solve single-particle Schrödinger equation, instead of constrained selfconsistent Hartree–Fock equations. It is shown, that it is possible to simulate one-dimensional three-center system via inverse scattering method in the approximatio...

We consider special patterns of lengths 5 and 6 in a ternary alphabet. We show that some of them are unavoidable in square-free words and prove avoidability of the other ones. Proving the main results, we use Fibonacci words as codes of ternary words in some natural coding system and show that they can be decoded to squarefree words avoiding the required patterns. Furthermore, we estimate the m...

in this work an analytical equation of state has been employed to calculate the pvt properties ofternary refrigerant mixtures. the theoretical eos is that of ihm, song and mason, which is based onstatistical-mechanical perturbation theory, and the two constants are enthalpy of vaporization δhvapand molar density ρnb, both at the normal boiling temperature. the following three temperaturedepende...

The likelihood that an undercooled liquid vitrifies or crystallizes depends on the cooling rate R. The critical cooling rate R(c), below which the liquid crystallizes upon cooling, characterizes the glass-forming ability (GFA) of the system. While pure metals are typically poor glass formers with R(c)>10(12)K/s, specific multi-component alloys can form bulk metallic glasses (BMGs) even at cooli...

The Bi–In–Sn–Zn system is an important alloy system in lead-free soldering. Thermodynamic descriptions for the ternary systems Bi–In–Sn, Bi–In–Zn, Bi–Sn–Zn and In–Sn–Zn are optimized, using the CALPHAD method and combined to obtain a description of the quaternary Bi–In–Sn–Zn. All available experimental data from the literature are taken into consideration in the optimization. Calculated liquidi...