The effect of a Buttiauxella phytase, in a dose range of 0-1000 FTU/kg in feed, on production performance in growing/finishing pigs fed European type wheat, corn, barley and SBM based diet was determined. Five dietary treatments were tested including a positive control (PC), a negative control (NC) without inorganic P and with reduced Ca (–0.12%) and metabolisable energy content (–0.14 MJ ME/kg...

The aim of this experiment was to compare the responses of young broiler chickens directly with the responses of turkeys to different dietary phytase concentrations. Nine hundred sixty birds (480 female Ross 308 broilers, and 480 female BUT6 turkeys) were reared in 64 floor pens from 0 to 21 d of age. Each species was fed a nutritionally complete (12.79 MJ/kg of AME, 231 g/kg of CP vs. 11.75 MJ...

Phytase, an enzyme that degrades the phosphorus storage compound phytate, has the potential to enhance phosphorus availability in animal diets when engineered into soybean (Glycine max) seeds. The phytase gene from Aspergillus niger was inserted into soybean transformation plasmids under control of constitutive and seed-specific promoters, with and without a plant signal sequence. Suspension cu...

An experiment was conducted to determine the influence of adding graded levels of microbial phytase to corn, distillers dried grains with solubles (DDGS), high-protein distillers dried grains (HP-DDG), and corn germ on the standardized total tract digestibility (STTD) of P. A second objective was to develop regression equations to predict the response of adding phytase to each of these ingredie...

A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide αfactor, under the control o...

Phytase and phytase gene from Aspergillus ficuum (A. ficuum) were used in this study. The results showed that phytase activity reached the peak of 0.17 U/g after 4 d incubation in solid medium for A. ficuum; the optimum pH and temperature of phytase were 2.5 and 50 o C, respectively. A 1.4-kb DNA containing the coding region of phytase gene was isolated and inserted into the expression vectors ...

There is considerable data on the effect of reducing inorganic Ca and P in broiler finisher diets on carcass quality. However, there is limited information on the effect of reducing dietary Ca and P during the different phases of growout. Two experiments were conducted from 0 to 35 d in floor pens. In both experiments, at least 4 replicates per treatment (50 chicks per replicate) were used. Cor...