High throughput screening has significantly contributed to advances in drug discovery. The great increase in the number of samples screened has been accompanied by increases in costs and in the data required for the investigated compounds. High throughput profiling addresses the issues of compound selectivity and specificity. It combines conventional screening with data mining technologies to g...

Accumulating evidence indicates that toll-like receptor 4 (TLR4) plays a critical role in promoting adaptive immune responses and are definitively involved in the expansion and maintenance of the neuropathic pain. However, the application of docking in virtual-screening in silico methods to drug discovery has some challenge but it allows us to make the directed and meaningful design of drugs fo...

This paper describes a high throughput screening architecture for functional genomics screens that use high content methods. Case studies were performed using the Yin case study approach. Additionally a detailed model is provided using the Method Engineering methodology to identify deficiencies. This study shows that current architectures lack interchangeability and functionality. © 2012 Publis...

With the exponential rise in the number of viable novel drug targets, computational methods are being increasingly applied to accelerate the drug discovery process. Virtual High Throughput Screening (vHTS) is one such established methodology to identify drug candidates from large collection of compound libraries. Although it complements the expensive and time consuming High Throughput Screening...

With the advances in biotechnology, identification of new therapeutic targets, and better un‐ derstanding of human diseases, pharmaceutical companies and academic institutions have accelerated their efforts in drug discovery. The pipeline to obtain therapeutics often involves target identification and validation, lead discovery and optimization, pre-clinical animal studies, and eventually clini...

Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Current methods, while highly developed and powerful, are falling short of their goal of routinely analyzing whole proteomes mainly because the wealth of proteomic information accumulated from prior studies is not used for the planning or interpretation of present experiments. ...

Small-molecule screening campaigns of model bacteria have been conducted extensively in biotechnology and pharmaceutical companies to search for novel compounds with antibacterial activity. Recently, there has been increasing interest in running such high-throughput screens within academic settings to answer questions in biology. In this respect, whole-cell screening has the particular advantag...

Due to its small size and optical transparency, zebrafish larvae are excellent vertebrate models to study human diseases in vivo. We previously developed a high throughput screening platform capable of handling, cellular-resolution imaging and optically manipulating zebrafish larvae. Here, we present new novel technologies to significantly increase the throughput of our screening platform by mu...

A versatile LED based miniature photobioreactor ideal for factorial experiments that allows simultaneous testing of a wide range variables and experimental combinations needed high-throughput screening analysis.