A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H₂O₂ at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 - 8.5×10-5 M and ...

This paper describes the development of a dual-channel microchip in a dual-parallel electrode configuration for voltammetric characterization of analytes in real time. The microchip system contains two distinct separation channels, each having a platinum working electrode set at a different potential. Two separate electropherograms were recorded and current ratios were generated based on the cu...

In this contribution a chip-integrated amperometric sensor for the detection of H2O2 in exhaled breath condensate (EBC) is reported. The electrode chip is characterized, and detection of H2O2 in an aqueous phase is shown by means of cyclic voltammetry (CV) and amperometry. Variation of conditions such as the composition of the supporting electrolyte largely influences the obtained electrochemic...

At any electrode immersed in an electrolyte solution, a specific interfacial region is formed. This region is called the double layer. The electrical properties of such a layer are important, since they significantly affect the electrochemical measurements. In an electrical circuit used to measure the current that flows at a particular working electrode, the double layer can be viewed as a capa...

Nano-Au and methylene blue (MB) were assembled layer-by-layer (LBL) into films on the glassy carbon electrode modified by thiourea for detection of human chorionic gonadotrophin (HCG). The electrode modification process was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron micrograph (SEM). The factors influencing the performance of the...

a rapid isocratic method of high performance liquid chromatography system (hplc) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (no2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. the solid extraction  reversed phase cartridges (sep-pak) are used for dep...

A new liquid membrane electrode for caffeine prepared from a solution of caffeine-picrylsulfonate ion-pair complex in n-octanol is developed. It exhibits Nernstian response in the range of w-·2 to 10M caffeine with a cationic slope of 59 mY/concentration decade. The electrode has a wide working pH range (5.59.5), fast response time (20 seconds1.5 minutes), stable response for at least 30 days a...

For a dye-sensitized solar cell with a near-infrared squaraine (SQ1) sensitizer, the photovoltaic performance was enhanced remarkably with a reflective luminescent down-shifting (R-LDS) layer to increase the light-harvesting efficiency at the wavelength region 400-550 nm where the SQ1 dye has weak absorption. Relative enhancements greater than 200% in IPCE near 500 nm and 40-54% in JSC were ach...

A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping beta-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATCh...

Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high se...