نتایج جستجو برای: zinc enriched yeast
تعداد نتایج: 219946 فیلتر نتایج به سال:
Mechanisms through which gene expression is regulated by zinc are central to cellular zinc homoeostasis. In this context, evidence for the involvement of zinc dyshomoeostasis in the aetiology of diseases, including Type 2 diabetes, Alzheimer's disease and cancer, highlights the importance of zinc-regulated gene expression. Mechanisms elucidated in bacteria and yeast provide examples of differen...
In vivo-applied sodium selenide or sodium selenite causes the appearance of zinc-selenium nanocrystals in places where free or loosely bound zinc ions are present. These nanocrystals can in turn be silver enhanced by autometallographic (AMG) development. The selenium method was introduced in 1982 as a tool for zinc-ion tracing, e.g., in vesicular compartments such as synaptic vesicles of zinc-e...
Two specific chemical oci have been implicated in the catalytic action of yeast alcohol dehydrogenase: sulfhydryl groups (2-5) and zinc (6, 7). The activity of this enzyme is inhibited by agents considered to interact with SH groups, e.g. iodoacetate (3)) iodosobenzoate (5)) iodoacetamide (5), trivalent arsenicals (4,5), N’-ethyhnaleimide,’ ultraviolet irradiation (8)) and certain metal ions: A...
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to create targeted mutations and enriched using a reporter plasmid system. The reporter expresses RFP and eGFP simultaneously when ZFN-mediated site-specific mutations occur. Thus, double positive cells...
Background and purpose: Stress is one of the decisive factors in infertility, which can interfere with spermatogenesis and reduce spermatozoa. Considering the immobilization stress-induced infertility disorders, this study aimed at examining the protective effect of selenium yeast cell wall (as an antioxidant) on in vitro fertilizing ability following immobilization stress in adult male rats. ...
A significant body of evidence shows that polyglutamine (polyQ) tracts are important for various biological functions. The characteristic polymorphism of polyQ length is thought to play an important role in the adaptation of organisms to their environment. However, proteins with expanded polyQ are prone to form amyloids, which cause diseases in humans and animals and toxicity in yeast. Saccharo...
We report a cell-free system that measures transport-coupled maturation of carboxypeptidase Y (CPY). Yeast spheroplasts are lysed by extrusion through polycarbonate filters. After differential centrifugation, a 125,000-g pellet is enriched for radiolabeled proCPY and is used as "donor" membranes. A 15,000-g pellet, harvested from nonradiolabeled cells and enriched for vacuoles, is used as "acce...
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